Cetinkaya A, Kantarceken B, Bulbuloglu E, Kurutas E B, Ciralik H, Atli Y
Bratisl Lek Listy. 2013;114(12):682-8. doi: 10.4149/bll_2013_145.
To investigate the effects of L-carnitine (LCAR) and N-acetylcysteine (NAC) on carbon tetrachloride (CCl4)-induced acute liver damage in rats.
Totally, 40 rats in 5 groups were included in the study. The first group was the control group. Group 2 received CCl4 (2 ml/kg). Group 3 was given CCl4 + NAC (150 mg/kg). The rats in the Group 4 were administered CCl4 + LCAR (100 mg/kg), and the rats in the Group 5 were given CCl4 + NAC + LCAR. Both CCl4 and the treatment protocols were administered via intraperitoneal route for 10 days. Tissue oxidative stress and antioxidant markers were investigated in liver tissue and serum liver enzymes were measured.
The levels of blood liver enzymes (ALT and AST) increased significantly in the Group 2. However, they decreased markedly in all treatment groups. While malondialdehyde and myeloperoxidase levels in the liver tissue samples increased significantly in the 2nd group, those levels were determined to be decreased significantly in all treatment groups. When the liver tissue antioxidant levels were evaluated; reduced glutathione and catalase decreased markedly in the 2nd group, but increased following the administration of NAC and LCAR. The activities of liver tissue superoxide dismutase did not differ significantly among the groups. In the histopathologic evaluation of liver tissues, on the other hand, diffuse hepatosteatosis was observed in all groups except the control group and there was no significant difference among the groups from the point of steatosis.
LCAR and NAC were concluded to have beneficial effects on the acute liver damage induced by CCl4 administration (Tab. 1, Fig. 5, Ref. 52). Text in PDF www.elis.sk.
研究左旋肉碱（LCAR）和N-乙酰半胱氨酸（NAC）对四氯化碳（CCl4）诱导的大鼠急性肝损伤的影响。
本研究共纳入5组40只大鼠。第一组为对照组。第二组给予CCl4（2 ml/kg）。第三组给予CCl4 + NAC（150 mg/kg）。第四组大鼠给予CCl4 + LCAR（100 mg/kg），第五组大鼠给予CCl4 + NAC + LCAR。CCl4及各治疗方案均通过腹腔注射给药，持续10天。检测肝组织中的组织氧化应激和抗氧化标志物，并测定血清肝酶。
第二组血肝酶（ALT和AST）水平显著升高。然而，在所有治疗组中这些水平均显著降低。第二组肝组织样本中的丙二醛和髓过氧化物酶水平显著升高，而在所有治疗组中这些水平均显著降低。评估肝组织抗氧化水平时；第二组中还原型谷胱甘肽和过氧化氢酶显著降低，但在给予NAC和LCAR后升高。肝组织超氧化物歧化酶的活性在各组之间无显著差异。另一方面，在肝组织的组织病理学评估中，除对照组外，所有组均观察到弥漫性肝脂肪变性，且各组之间在脂肪变性方面无显著差异。
得出结论认为LCAR和NAC对CCl4给药诱导的急性肝损伤具有有益作用（表1，图5，参考文献52）。PDF文本www.elis.sk 。
