Zhu Chenggang, Ge Bilin, Chen Ru, Zhu Xiangdong, Mi Lan, Ma Jiong, Wang Xu, Zheng Fengyun, Fei Yiyan
Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai 200433, China.
Department of Physics, University of California, Davis, CA 95616, USA.
Sensors (Basel). 2018 Feb 9;18(2):524. doi: 10.3390/s18020524.
Total internal reflection (TIR) is useful for interrogating physical and chemical processes that occur at the interface between two transparent media. Yet prism-coupled TIR imaging microscopes suffer from limited sensing areas due to the fact that the interface (the object plane) is not perpendicular to the optical axis of the microscope. In this paper, we show that an electrically tunable lens can be used to rapidly and reproducibly correct the focal length of an oblique-incidence scanning microscope (OI-RD) in a prism-coupled TIR geometry. We demonstrate the performance of such a correction by acquiring an image of a protein microarray over a scan area of 4 cm² with an effective resolution of less than 20 microns. The electronic focal length tuning eliminates the mechanical movement of the illumination lens in the scanning microscope and in turn the noise and background drift associated with the motion.
全内反射（TIR）对于研究发生在两种透明介质界面处的物理和化学过程很有用。然而，棱镜耦合全内反射成像显微镜由于界面（物平面）不垂直于显微镜的光轴，其传感区域有限。在本文中，我们表明电可调透镜可用于在棱镜耦合全内反射几何结构中快速且可重复地校正斜入射扫描显微镜（OI-RD）的焦距。我们通过在4平方厘米的扫描区域上获取蛋白质微阵列的图像，有效分辨率小于20微米，来展示这种校正的性能。电子焦距调谐消除了扫描显微镜中照明透镜的机械运动，进而消除了与该运动相关的噪声和背景漂移。
