一种用于细胞分析的电化学表面等离子体共振成像系统。

An electrochemical surface plasmon resonance imaging system targeting cell analysis.

作者信息

Zhang L L, Chen X, Wei H T, Li H, Sun J H, Cai H Y, Chen J L, Cui D F

机构信息

State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, China.

出版信息

Rev Sci Instrum. 2013 Aug;84(8):085005. doi: 10.1063/1.4819027.

DOI:10.1063/1.4819027

PMID:24007100

Abstract

This paper presents an electrochemical-surface plasmon resonance imaging (EC-SPRI) system, enabling the characterization of optical and electrical properties of cells, simultaneously. The developed surface plasmon resonance (SPR) imaging system was capable of imaging micro cavities with a dimension of 10 μm × 10 μm and differentiated glycerol solutions with a group of refractive indices (RIs). Furthermore, the EC-SPRI system was used to image A549 cells, suggesting corresponding RI and morphology changes during the cell death process. In the end, electrochemical and SPR methods were used in combination, recording oxidation peaks of A549 cells in the cyclic voltage curves and SPR response unit increase, simultaneously.

摘要

本文介绍了一种电化学表面等离子体共振成像（EC-SPRI）系统，该系统能够同时表征细胞的光学和电学性质。所开发的表面等离子体共振（SPR）成像系统能够对尺寸为10μm×10μm的微腔进行成像，并区分具有一组折射率（RI）的甘油溶液。此外，EC-SPRI系统用于对A549细胞进行成像，显示了细胞死亡过程中相应的折射率和形态变化。最后，将电化学方法和SPR方法结合使用，同时记录循环电压曲线中A549细胞的氧化峰和SPR响应单位的增加。