University of Kragujevac, Faculty of Agronomy, Department of Food Technology, Cara Dušana 34, 32000 Čačak, Serbia.
University of Kragujevac, Faculty of Science, Department of Chemistry, Radoja Domanovića 12, 34000 Kragujevac, Serbia.
Phytomedicine. 2018 Jan 1;38:118-124. doi: 10.1016/j.phymed.2017.11.010. Epub 2017 Nov 29.
Lavatera thuringiaca L. is herbaceous perennial plant from Malvaceae family, which is known for its biological activity and richness in polyphenolic compounds. Despite this, the information regarding the biological activity and chemical profile is still insufficient.
Aim of this study was to investigate biological potential and chemical profile of Lavatera thuringiaca L., as well as influence of applied extraction technique on them.
Two conventional and four non-conventional extraction techniques were applied in order to obtain extracts rich in bioactive compound. Extracts were further tested for total phenolics, flavonoids, condensed tannins, gallotannins and anthocyanins contents using spectrophotometric assays. Polyphenolic profile was established using HPLC-DAD analysis. Biological activity was investigated regarding antioxidant, cytotoxic and antibacterial activities. Four antioxidant assays were applied as well as three different cell lines for cytotoxic and fifteen bacterial strain for antibacterial activity.
Results showed that subcritical water extraction (SCW) dominated over the other extraction techniques, where SCW extract exhibited the highest biological activity. Study indicates that plant Lavatera thuringiaca L. may be used as a potential source of biologically compounds.
Lavatera thuringiaca L. 是锦葵科的一种多年生草本植物，以其生物活性和富含多酚化合物而闻名。尽管如此，关于其生物活性和化学特征的信息仍然不足。
本研究旨在研究 Lavatera thuringiaca L. 的生物潜力和化学特征，以及所采用的提取技术对其的影响。
为了获得富含生物活性化合物的提取物，应用了两种常规和四种非常规提取技术。使用分光光度法测定提取物中总酚、类黄酮、缩合单宁、没食子单宁和花青素的含量，进一步测试提取物的含量。使用 HPLC-DAD 分析建立多酚特征。抗氧化、细胞毒性和抗菌活性。应用了四种抗氧化测定方法，以及三种不同的细胞系进行细胞毒性测定和十五种细菌菌株进行抗菌活性测定。
结果表明，亚临界水提取（SCW）优于其他提取技术，SCW 提取物表现出最高的生物活性。研究表明，植物 Lavatera thuringiaca L. 可能被用作生物化合物的潜在来源。
