Jevtovic Violeta, Alabbosh Khulood Fahad Saud, Alyami Reem Ali, Alshammari Maha Raghyan, Alshammari Badriah, Mašković Pavle, Mašković Jelena, Nikolić Jelena, Mitić Milan
Chemistry Department, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia.
Biology Department, College of Science, University of Ha'il, Ha'il 81451, Saudi Arabia.
Plants (Basel). 2025 May 30;14(11):1679. doi: 10.3390/plants14111679.
The effects of temperature, time, and solvent concentration on the yield of bioactive compounds (BCs) with cytotoxic activity against three cell lines (Hep2c, RD, and L2OB) from L., as well as their influence on kinetic and thermodynamic parameters (enthalpy, entropy, and free energy), were investigated. The extract obtained at 30% ethanol, 50 °C, and 80 min showed the highest cytotoxic activity with IC values of 14.29, 13.93, and 22.23 µg/mL, respectively. The kinetics of BC extraction with cytotoxic activity was better described by the unsteady-state diffusion model compared to the Ponomarev model. The activation energy obtained was in the range of = (4.92-26.57) kJ/mol. The thermodynamic parameters under transition theory at 50 °C were ∆S* = (-220.22)-(-285.15) J/Kmol, ∆H* = (2.24-23.88) kJ/mol, and ∆G* = (94.34-96.30) kJ/mol, indicating that the extraction of BCs with cytotoxic activity against the three cell lines is an irreversible and endothermic process. In addition to cytotoxic activity, the extracts demonstrated strong antioxidant activity (e.g., DPPH IC = 16.55 µg/mL) and antibacterial activity against multiple bacterial strains. Five antioxidant assays were applied, along with tests against eight bacterial strains for antibacterial activity. These findings suggest that L. is a promising natural source of multifunctional bioactive compounds with therapeutic potential.
研究了温度、时间和溶剂浓度对来自L.的具有针对三种细胞系（Hep2c、RD和L2OB）的细胞毒性活性的生物活性化合物（BCs）产量的影响，以及它们对动力学和热力学参数（焓、熵和自由能）的影响。在30%乙醇、50°C和80分钟条件下获得的提取物显示出最高的细胞毒性活性，IC值分别为14.29、13.93和22.23μg/mL。与Ponomarev模型相比，非稳态扩散模型能更好地描述具有细胞毒性活性的BCs提取动力学。获得的活化能范围为 = (4.92 - 26.57) kJ/mol。在50°C下，过渡理论下的热力学参数为∆S* = (-220.22)-(-285.15) J/Kmol，∆H* = (2.24 - 23.88) kJ/mol，∆G* = (94.34 - 96.30) kJ/mol，表明针对这三种细胞系具有细胞毒性活性的BCs提取是一个不可逆的吸热过程。除细胞毒性活性外，提取物还表现出较强的抗氧化活性（例如，DPPH IC = 16.55μg/mL）和对多种细菌菌株的抗菌活性。应用了五种抗氧化测定方法，以及针对八种细菌菌株的抗菌活性测试。这些发现表明L.是一种具有治疗潜力的多功能生物活性化合物的有前景的天然来源。
