School of Food Science and Engineering, Hefei University of Technology, Hefei, PR China; Department of Chemical Engineering and Food Processing, Xuancheng Campus,, Hefei University of Technology, Xuancheng, PR China.
School of Food Science and Engineering, Hefei University of Technology, Hefei, PR China; Department of Chemical Engineering and Food Processing, Xuancheng Campus,, Hefei University of Technology, Xuancheng, PR China.
Food Chem Toxicol. 2018 Sep;119:375-379. doi: 10.1016/j.fct.2018.02.008. Epub 2018 Feb 6.
Phenolic acids and flavonoids in bamboo leaves are of great importance for their functional attributes, but they can hardly be separated simultaneously. In this study, zirconia was prepared and applied as a potential absorbent for simultaneous separation of these phenolic compounds. Three phenolic acids (neochlorogenic acid, chlorogenic acid and cryptochlorogenic acid) and two flavonoids (isoorientin and orientin) were isolated at the same time. The influence of bamboo leaves extraction conditions, zirconia calcination temperatures, desorption conditions and absorption/desorption dynamics on the separation were further investigated. When zirconia-400 (calcined at 400 °C) was treated with 70% ethanol extract of bamboo leaves for 40 min followed by desorption with 70% acetic acid solution for 60 min, the recovery of three chlorogenic acids and two flavonoids was about 65%. To conclude, the concise method developed here may provide a new way for simultaneous separation of phenolic acids and flavonoids from various plants.
竹叶中的酚酸和类黄酮因其功能特性而非常重要，但它们很难同时分离。在这项研究中，氧化锆被制备并用作同时分离这些酚类化合物的潜在吸附剂。同时分离出三种酚酸（新绿原酸、绿原酸和隐绿原酸）和两种类黄酮（异荭草苷和荭草苷）。进一步研究了竹叶提取条件、氧化锆煅烧温度、解吸条件和吸附/解吸动力学对分离的影响。当用 70%乙醇处理竹叶提取物 40 分钟，然后用 70%乙酸溶液解吸 60 分钟时，三种绿原酸和两种类黄酮的回收率约为 65%。总之，这里开发的简洁方法可能为从各种植物中同时分离酚酸和类黄酮提供一种新途径。
