College of New Energy and Environmental Engineering, Nanchang Institute of Technology, Nanchang, China.
Jiangxi Key Laboratory of Natural Product and Functional Food College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, China.
Nat Prod Res. 2020 Jul;34(14):2076-2079. doi: 10.1080/14786419.2019.1569013. Epub 2019 Feb 5.
The chemical profile of cv. () leaves was analysed by UPLC-DAD-Q-TOF-MS. Twelve compounds were identified and -glycosyl flavonoids, including vitexin, isovitexin, isoorientin and its derivatives, are the main constitutes of the plant. Besides, a HPLC method for isoorientin quantification was developed. The RSD of retention time and peak area were 0.05% and 2.04% for six times analysis of isoorientin with concentration of 20 μg/mL. The recovery of isoorientin in real sample was 99.2%. The general trend of isoorientin content in leaves was that it steady increased from Jan. to May, and then quickly decreased. The maximum was found on May with value of 4.7 mg/g. The lowest level of isoorientin was found during Aug. to Nov. with value of about 1.66 mg/g. In different seasons, isoorientin is always the most dominant flavonoid which was accounted for about 50% of total flavonoids in the sample.
利用 UPLC-DAD-Q-TOF-MS 对 cv. （）叶的化学成分进行了分析。鉴定出 12 种化合物，-糖苷黄酮类化合物，包括牡荆素、异牡荆素、异荭草素及其衍生物，是该植物的主要成分。此外，还建立了一种用于异荭草素定量分析的 HPLC 方法。浓度为 20μg/mL 时，异荭草素的保留时间和峰面积的 RSD 分别为 0.05%和 2.04%，六次分析的重复性良好。异荭草素在实际样品中的回收率为 99.2%。异荭草素在 cv. （）叶中的含量总体上呈现出从 1 月到 5 月稳定增加，然后迅速下降的趋势。5 月含量最高，为 4.7mg/g。8 月到 11 月含量最低，约为 1.66mg/g。在不同的季节，异荭草素始终是最主要的类黄酮，占样品总类黄酮的 50%左右。
