Seo Chang-Seob, Song Kwang-Hoon
KM Science Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Korea.
KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Korea.
Plants (Basel). 2021 Dec 24;11(1):50. doi: 10.3390/plants11010050.
leaves are cultivated in a number of Asian countries and have been used for antipyretic and diuretic effects since ancient times, especially in Korea. The purpose of this study was to develop and validate of analytical method for quality control of leaves using high-performance liquid chromatography with diode array detector (HPLC-DAD) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) detection. HPLC-DAD analysis was conducted with a Gemini C column, and distilled water-acetonitrile (both with 0.1% () formic acid) mobile-phase system. For the LC-MS/MS analysis, all markers were separated with a Waters ACQUITY UPLC BEH C column and gradient flow system of distilled water containing 0.1% () formic acid and 5 mM ammonium formate-acetonitrile. In both method, major components were detected at 2.13-11.63 mg/g (HPLC-DAD) and 0.12-19.20 mg/g (LC-MS/MS). These methods were validated with respect to linearity (coefficient of determination >0.99), recovery (95.22-118.81%), accuracy (90.52-116.96), and precision (<4.0%), and were successfully applied for the quantitative analysis of leaves.
这种叶子在一些亚洲国家种植，自古以来就被用于解热和利尿，尤其是在韩国。本研究的目的是开发并验证一种使用二极管阵列检测器的高效液相色谱法（HPLC-DAD）和串联质谱检测的液相色谱法（LC-MS/MS）对这种叶子进行质量控制的分析方法。HPLC-DAD分析使用Gemini C柱，流动相系统为蒸馏水 - 乙腈（均含有0.1%（）甲酸）。对于LC-MS/MS分析，所有标志物在含有0.1%（）甲酸和5 mM甲酸铵的蒸馏水 - 乙腈的Waters ACQUITY UPLC BEH C柱和梯度流动系统中分离。在这两种方法中，主要成分的检测含量分别为2.13 - 11.63 mg/g（HPLC-DAD）和0.12 - 19.20 mg/g（LC-MS/MS）。这些方法在线性（决定系数>0.99）、回收率（95.22 - 118.81%）、准确度（90.52 - 116.96）和精密度（<4.0%）方面得到了验证，并成功应用于这种叶子的定量分析。
需注意，原文中括号处内容不完整，翻译时保留原样。
