Messenger A J, Hall R M, Ratledge C
J Gen Microbiol. 1986 Mar;132(3):845-52. doi: 10.1099/00221287-132-3-845.
The production of exochelins (MV) was established in Mycobacterium vaccae R877R under iron-deficient conditions in concentrations about five times greater than in Mycobacterium smegmatis. M. vaccae does not produce mycobactin nor is salicylic acid secreted into the medium. A simple method is described using 55Fe-labelled culture filtrates for assessing exochelin production and which would be applicable to other mycobacteria. One of the exochelins produced (MV3) is part of an active iron uptake system and another (MV1) is responsible for a passive uptake system. MV3 exochelin has similar chromatographic properties and biological activity to the major exochelin produced by M. smegmatis: iron uptake from MV3 exochelin was inhibited by dinitrophenol, NaN3 and HgCl2, and was judged to be an active transport process. This process was not inhibited by equimolar amounts of ferri-salicylate or ferri-citrate both of which could be used separately as sources of iron for the organism. Uptake from these latter sources was insensitive to metabolic inhibitors and uncouplers. The multiplicity of pathways for iron uptake in a single organism is discussed.
在缺铁条件下，母牛分枝杆菌R877R中可产生外螯合铁（MV），其产量约为耻垢分枝杆菌的五倍。母牛分枝杆菌不产生分枝杆菌素，也不向培养基中分泌水杨酸。本文描述了一种使用55Fe标记的培养滤液评估外螯合铁产生的简单方法，该方法也适用于其他分枝杆菌。所产生的一种外螯合铁（MV3）是活跃铁摄取系统的一部分，另一种（MV1）负责被动摄取系统。MV3外螯合铁具有与耻垢分枝杆菌产生的主要外螯合铁相似的色谱性质和生物活性：MV3外螯合铁的铁摄取受到二硝基苯酚、NaN3和HgCl2的抑制，被判定为一个主动运输过程。该过程不受等摩尔量的水杨酸铁或柠檬酸铁的抑制，这两种物质均可单独用作该生物体的铁源。从后一种铁源的摄取对代谢抑制剂和解偶联剂不敏感。本文讨论了单一生物体中铁摄取途径的多样性。
