分段式莲子抗性淀粉的结构性质和益生元活性。
Structural properties and prebiotic activities of fractionated lotus seed resistant starches.
机构信息
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
出版信息
Food Chem. 2018 Jun 15;251:33-40. doi: 10.1016/j.foodchem.2018.01.057. Epub 2018 Jan 6.
The objective of this study was to fractionate lotus seed resistant starch (LRS3) and investigate their structural properties and prebiotic activities. Two main fractions of resistant starch precipitated gradually by ethanol at concentrations of 20% and 30% were named as LRS3-20% and LRS3-30%, respectively. The swelling power and solubility of LRS3-20% were smaller compared to LRS3-30%, and their moisture and resistant starch contents were not significantly different. LRS3-20% and LRS3-30% had molecular weights mainly of 2.0 × 10-4.0 × 10 and 1.0 × 10-2.0 × 10 g/mol. Layered strips and gully shapes were evident on the rough surfaces of LRS3-20%, while LRS3-30% displayed a relatively smooth surface. Both LRS3-20% and LRS3-30% had a B-type crystalline structure with LRS3-20% containing more ordered structures and double-helices. Furthermore, LRS3-20% displayed higher prebiotic activities against Bifidobacterium adolescentis and Lactobacillus acidophilus compared to LRS3-30% and high amylose maize starch. This effect was related to its rough surface and double helix structure.
本研究旨在分离莲子抗性淀粉（LRS3），并研究其结构特性和益生元活性。通过乙醇逐渐沉淀出两种主要的抗性淀粉，浓度分别为 20%和 30%，分别命名为 LRS3-20%和 LRS3-30%。与 LRS3-30%相比，LRS3-20%的膨胀力和溶解度较小，其水分和抗性淀粉含量无显著差异。LRS3-20%和 LRS3-30%的分子量主要为 2.0×10-4.0×10 和 1.0×10-2.0×10 g/mol。LRS3-20%表面粗糙，有层状条带和沟壑，而 LRS3-30%表面相对光滑。LRS3-20%和 LRS3-30%均具有 B 型结晶结构，LRS3-20%含有更多有序结构和双螺旋。此外，与 LRS3-30%和高直链玉米淀粉相比，LRS3-20%对青春双歧杆菌和嗜酸乳杆菌具有更高的益生元活性。这种作用与其粗糙的表面和双螺旋结构有关。
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