Ma Jian, Cao Bixuan, Chen Xiu, Xu Minjuan, Bi Xiaoxu, Guan Peipei, Jiang Yi, Xu Jun, Han Li, Huang Xueshi
Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110819, PR China.
Yunnan Institute of Microbiology, School of Life Science, Yunnan University, Kunming 650091, PR China.
Bioorg Med Chem Lett. 2018 Mar 1;28(5):947-951. doi: 10.1016/j.bmcl.2018.01.051. Epub 2018 Jan 31.
A new chromanone derivative, named violacin A (1), was isolated from the fermentation broth of Streptomyces violaceoruber as a potential anti-inflammatory compound. The structure of violacin A was established using comprehensive NMR spectroscopic data analysis together with UV, IR, and MS data. The anti-inflammatory effects and action mechanisms of violacin A were investigated in vitro. The results demonstrated that violacin A attenuated the production of NO, IL-1β, IL-6, and TNF-α as well as inhibited the expression of iNOS in LPS-induced RAW 264.7 cells. Additionally, Western blot and qRT-PCR results revealed that 1 down-regulated pro-inflammatory cytokines expression correlated with the suppression of NF-κB signaling pathway.
从紫色链霉菌的发酵液中分离出一种名为紫红素A（1）的新型色满酮衍生物，它是一种潜在的抗炎化合物。通过综合的核磁共振光谱数据分析以及紫外、红外和质谱数据确定了紫红素A的结构。在体外研究了紫红素A的抗炎作用及其作用机制。结果表明，紫红素A可减轻脂多糖诱导的RAW 264.7细胞中一氧化氮、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α的产生，并抑制诱导型一氧化氮合酶的表达。此外，蛋白质免疫印迹和定量逆转录聚合酶链反应结果显示，紫红素A下调促炎细胞因子的表达与抑制核因子-κB信号通路相关。
