Idris Hamidah, Nouioui Imen, Pathom-Aree Wasu, Castro Jean Franco, Bull Alan T, Andrews Barbara A, Asenjo Juan A, Goodfellow Michael
School of Natural and Environmental Sciences, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Biology Department, Sultan Idris Education University, 35900, Tanjung Malim, Perak, Malaysia.
Antonie Van Leeuwenhoek. 2018 Sep;111(9):1523-1533. doi: 10.1007/s10482-018-1039-3. Epub 2018 Feb 10.
The taxonomic position of a novel Amycolatopsis strain isolated from a high altitude Atacama Desert subsurface soil was established using a polyphasic approach. The strain, isolate H5, was shown to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the diamino acid in the cell wall peptidoglycan, arabinose and galactose as diagnostic sugars and MK-9(H) as the predominant isoprenologue. It also has cultural and morphological properties consistent with its classification in the genus, notably the formation of branching substrate hyphae which fragment into rod-like elements. 16S rRNA gene sequence analyses showed that the strain is closely related to the type strain of Amycolatopsis mediterranei but could be distinguished from this and other related Amycolatopsis strains using a broad range of phenotypic properties. It was separated readily from the type strain of Amycolatopsis balhymycina, its near phylogenetic neighbour, based on multi-locus sequence data, by low average nucleotide identity (92.9%) and in silico DNA/DNA relatedness values (51.3%) calculated from draft genome assemblies. Consequently, the strain is considered to represent a novel species of Amycolatopsis for which the name Amycolatopsis vastitatis sp. nov. is proposed. The type strain is H5 (= NCIMB 14970 = NRRL B-65279).
采用多相分类法确定了从阿塔卡沙漠高海拔地下土壤中分离出的一株新的拟无枝酸菌菌株的分类地位。该菌株（分离株H5）具有拟无枝酸菌属成员典型的化学性质，如细胞壁肽聚糖中的二氨基酸为内消旋二氨基庚二酸，诊断糖为阿拉伯糖和半乳糖，主要类异戊二烯醌为MK-9(H)。它还具有与其在该属中的分类一致的培养和形态学特征，特别是形成分支的基质菌丝，并断裂成杆状元件。16S rRNA基因序列分析表明，该菌株与地中海拟无枝酸菌模式菌株密切相关，但可通过广泛的表型特征与该菌株及其他相关拟无枝酸菌菌株区分开来。基于多位点序列数据，通过从草图基因组组装计算得到的低平均核苷酸同一性（92.9%）和电子DNA/DNA相关性值（51.3%），它很容易与近缘系统发育邻居巴利拟无枝酸菌模式菌株区分开来。因此，该菌株被认为代表了拟无枝酸菌属的一个新物种，为此提出了新名称阿塔卡拟无枝酸菌（Amycolatopsis vastitatis sp. nov.）。模式菌株为H5（=NCIMB 14970=NRRL B-65279）。
