School of Natural and Environmental Sciences, Ridley Building 2, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
Departamento de Microbiología y Genética, Edificio Departamental, Lab. 214, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain.
Int J Syst Evol Microbiol. 2019 Nov;69(11):3426-3436. doi: 10.1099/ijsem.0.003634.
A strain, designated 5R2A7, isolated from a high altitude Atacama Desert soil was examined by using a polyphasic approach. Strain 5R2A7 was found to have morphological, chemotaxonomic and cultural characteristics typical of members of the genus The cell wall contains - and -diaminopimelic acid, the major whole-cell sugars are glucose, ribose and xylose, the predominant menaquinones MK-10(H), MK-10(H), MK-10(H) and MK-9(H), the major polar lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown glycolipid, and the predominant cellular fatty acids -C, -C and 10-methyl C. The digital genomic DNA G+C content is 72.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain 5R2A7 was closely related to DSM 44875 (99.8 %) and CR30 (99.7 %), and was separated readily from the latter, its closest phylogenetic neighbour, based on and multilocus sequence data, by low average nucleotide identity (92.59 %) and DNA-DNA relatedness (51.7 %) values calculated from draft genome assemblies and by a range of chemotaxonomic and phenotypic properties. Consequently, strain 5R2A7 is considered to represent a novel species of for which the name sp. nov. is proposed. The type strain is 5R2A7 (=LMG 30755=CECT 9656).
一株从高海拔阿塔卡马沙漠土壤中分离得到的菌株 5R2A7，通过多相分类方法进行了研究。菌株 5R2A7 具有形态学、化学分类学和培养特征，典型地属于 属。其细胞壁含有 - 和 -二氨基庚二酸，主要的全细胞糖为葡萄糖、核糖和木糖，主要的menaquinones 为 MK-10(H)、MK-10(H)、MK-10(H)和 MK-9(H)，主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和一种未知的糖脂，主要的细胞脂肪酸为 -C、-C 和 10-甲基 C。基因组 DNA G+C 含量为 72.3 mol%。16S rRNA 基因序列的系统发育分析表明，菌株 5R2A7 与 DSM 44875（99.8%）和 CR30（99.7%）密切相关，与其最近的亲缘关系菌株 CR30 可以通过较低的平均核苷酸同一性（92.59%）和 DNA-DNA 同源性（51.7%）值，以及从基因组草图计算得到的和多位点序列数据，以及一系列化学分类学和表型特征进行区分。因此，菌株 5R2A7 被认为代表了 属的一个新种，提议命名为 sp. nov.。其模式菌株为 5R2A7（=LMG 30755=CECT 9656）。
