Bai L, Li W W, Han H H, Liu J K, Fu P, Zhang X L, Guo Y C
Key Laboratory of Food Safety Risk Assessment of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2018 Feb 6;52(2):124-128. doi: 10.3760/cma.j.issn.0253-9624.2018.02.002.
Tests were carried out for obtaining contamination level and antimicrobial resistance of on broiler carcasses after chilling in four poultry slaughterhouses in Henan. Totally, two hundred sixty nine broiler carcasses after chilling were collected in four slaughterhouses with the daily slaughter amount around 15 000 to 50 000. For qualitative analysis of EFSA method was used and for quantitative analysis of modified Rappaport-Vassiliadis most probable number (MSRV-MPN) method was used. All of the isolates were subjected to antimicrobial susceptibility testing of 8 antibiotics by minimum inhibitory concentration (MIC). Overall, 48.7% (131/269) of the broiler carcasses after chilling were contaminated by , and the average of contamination level is 1.32 most probable number MPN/g. Eight serotypes were detected. The dominant serotype is enteritidis (93, 71.0%) followed by Indiana (21, 16.0%). Only 2 (1.5%) enteritidis strains were sensitive to all the tested antibiotics and the remaining 129 isolates were resistant to at least one kind of eight class antibiotics. Among them, resistant to NAL was the common (104, 79.4%) and 51 (38.9%) isolates were multidrug-resistant. The contamination rate and multiple antimicrobial resistant of on broiler carcasses after chilling from slaughterhouses was very serious, while the isolates contained various serotypes.
对河南四家家禽屠宰场冷藏后的肉鸡胴体进行了检测,以获取[具体细菌名称未给出]的污染水平和抗菌耐药性。总共从四家屠宰场收集了269份冷藏后的肉鸡胴体,日屠宰量约为15000至50000只。对于[具体细菌名称未给出]的定性分析,采用了欧洲食品安全局(EFSA)的方法;对于定量分析,采用了改良的Rappaport-Vassiliadis最可能数(MSRV-MPN)法。所有分离株均通过最低抑菌浓度(MIC)对8种抗生素进行了药敏试验。总体而言,48.7%(131/269)的冷藏后肉鸡胴体被[具体细菌名称未给出]污染,污染水平的平均值为1.32最可能数MPN/g。检测到8种血清型。优势血清型是肠炎[具体细菌名称未给出](93株,71.0%),其次是印第安纳[具体细菌名称未给出](21株,16.0%)。只有2株(1.5%)肠炎[具体细菌名称未给出]菌株对所有测试抗生素敏感,其余129株分离株对至少一种八类抗生素耐药。其中,对萘啶酸(NAL)耐药最为常见(104株,79.4%),51株(38.9%)[具体细菌名称未给出]分离株为多重耐药。屠宰场冷藏后肉鸡胴体上[具体细菌名称未给出] 的污染率和多重抗菌耐药情况非常严重,且分离株包含多种血清型。