[Prevalence and antimicrobial susceptibility of isolated from broiler whole production process in four provinces of China].

To determine the prevalence and antimicrobial susceptibility of Salmonella isolated from broiler production process in 4 provinces of China. Using convenience sampling method, 238 sample sites from broiler whole production process were chosen in Henan, Jiangsu, Heilongjiang and Shandong provinces in 2012. A total of 11 592 samples were collected and detected to analyze prevalence baseline, including 2 090 samples from breeding chicken farms and hatcheries, 1 421 samples from broiler farms, 5 610 samples from slaughterhouses and 2 471 samples from distribution and retail stores. All strains were isolated through selective enrichment, and were serotyped according to Kauffmann-White scheme. The antimicrobial susceptibilities of selected strains were determined by the broth microdilution method and fourteen antimicrobial agents were examined. During incubation course, the average prevalence of was 5.5% in feces of breeding hens, feces of chicks, and hatching eggs, 123 Salmonella strains were isolated. During cultivation course, the prevalence of was 8.0% in feces from broiler farms, soil, feed, and workers, 114 strains were isolated. During slaughter course, the prevalence of was 24.9% in swabs pre-slaughter, dressed broiler carcasses, pre-cooled broiler carcasses, water from precooling pool, cutter and chipping boards, frozen chicken portions, and workers, 1 438 strains were isolated. During distribution and sale course, the prevalence of was 20.9% in transport carts, frozen chicken portions, retail chicken portions and workers, 551 strains were isolated. The dominant serotypes were Enteritidis (1 229) and Salmonella Indiana (621). Among 1 231 examined strains, 97.2% isolates were resistant to at least one antimicrobial, 69.9% strains were multi-drug resistant isolates. Our findings indicated that contamination was common and serious in commercial broiler whole production process in China, especially in the course of defeathering, precooling and selling. The environment of broiler farm is the important source of contamination. Additionally, antibiotic resistance of was serious for common antimicrobials and multi-drug resistant strains existed widespread, which can pose potential risk on public health and clinical therapy.