College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Integrate Microbiology Research Center, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.
J Hazard Mater. 2018 Apr 5;347:341-348. doi: 10.1016/j.jhazmat.2018.01.023. Epub 2018 Jan 11.
Recalcitrance limits biomass application in biorefinery. It is even more so when toxic chlorophenols are present. In this study, we screened a microbial consortium, OEM2, for lignocellulose deconstruction and chlorophenols detoxification through a short-term and efficient screening process. Microbial consortium OEM2 had a good buffer capability in the cultivation process and exhibited a high xylanase activity, with over 85% hemicellulose degradation within 12 days. Throughout the treatment process, 41.5% rice straw decomposition on day 12 and around 75% chlorophenols (MCP, 2,4-DCP, 2,4,6-TCP) removal on day 9, were recorded. Moreover, Fourier translation infrared spectroscopy (FTIR) analysis indicated that chemical bonds and groups (eg. hydrogen-bond, β-1,4 glycosidic bond, lignin-carbohydrate cross-linking) in the rice straw were broken. Cuticle and silica layer destruction and subsequent exposed cellulose fibers were observed by scanning electron microscopy (SEM). Microbial consortium OEM2 diversity analysis by 16S rRNA gene sequencing indicated that Proteobacteria (41.3%) was the most abundant phylum and the genera Paenibacillus and Pseudomonas played an important role in the lignocellulose decomposition and chlorophenols detoxification. This study developed a faster and more efficient strategy to screen a specific microbial consortium. And the new microbial consortium, OEM2, makes lignocellulose more accessible and complex pollutants unproblematic in the further biorefinery process.
顽固性限制了生物量在生物炼制中的应用。当存在有毒的氯酚时，情况更是如此。在这项研究中，我们通过短期、高效的筛选过程，筛选了一个微生物群落 OEM2，用于木质纤维素的解构和氯酚的解毒。微生物群落 OEM2 在培养过程中有良好的缓冲能力，并且表现出高木聚糖酶活性，在 12 天内超过 85%的半纤维素被降解。在整个处理过程中，第 12 天记录到 41.5%的稻草分解，第 9 天记录到约 75%的氯酚（MCP、2,4-DCP、2,4,6-TCP）去除。此外，傅里叶变换红外光谱（FTIR）分析表明，稻草中的化学键和基团（例如氢键、β-1,4 糖苷键、木质素-碳水化合物交联）被打破。扫描电子显微镜（SEM）观察到表皮和二氧化硅层的破坏以及随后暴露的纤维素纤维。通过 16S rRNA 基因测序对微生物群落 OEM2 的多样性分析表明，变形菌门（41.3%）是最丰富的门，芽孢杆菌属和假单胞菌属在木质纤维素分解和氯酚解毒中发挥了重要作用。本研究开发了一种更快、更有效的筛选特定微生物群落的策略。新型微生物群落 OEM2 使木质纤维素更容易获得，复杂污染物在进一步的生物炼制过程中也不再成问题。
