Lappe R W, Dinish J L, Bex F, Michalak K, Wendt R L
Pharmacol Biochem Behav. 1986 Jun;24(6):1573-6. doi: 10.1016/0091-3057(86)90487-9.
The present study examined the effects of ANF(4-28) [Wy-47,663], a synthetic 25 amino acid human atrial natriuretic factor, on the dipsogenic actions of centrally-administered angiotensin II in conscious rats. Bolus injection (100 ng) or continuous infusion (60 ng/min) of Wy-47,663 or vehicle into the lateral cerebroventricle had no effect on mean arterial pressure or heart rate. No obvious behavioral changes were observed after central administration of Wy-47,663 or vehicle. Central injection of angiotensin II (15 or 30 ng) promptly elicited prolonged drinking responses in vehicle-treated rats. In rats pretreated with Wy-47,663, the onset of the angiotensin II-induced drinking responses was significantly delayed compared to vehicle-treated animals. However, Wy-47,663 had no effect on the total volume consumed over 30 minutes after angiotensin II injection. Intravenous infusion of Wy-47,663 (2 micrograms/kg/min) failed to alter the dipsogenic action of centrally administered angiotensin II. These data indicate that atrial natriuretic factor found within the brain but not the peripheral circulation may participate in the regulation of extracellular fluid volume by modulating the dipsogenic actions of the central renin-angiotensin system.
本研究检测了人工合成的含25个氨基酸的人心房利钠因子ANF(4 - 28)[Wy - 47,663]对清醒大鼠中枢给予血管紧张素II的致渴作用的影响。向侧脑室推注(100 ng)或持续输注(60 ng/分钟)Wy - 47,663或赋形剂，对平均动脉压或心率无影响。中枢给予Wy - 47,663或赋形剂后，未观察到明显的行为变化。中枢注射血管紧张素II(15或30 ng)能迅速引起接受赋形剂处理的大鼠出现长时间的饮水反应。与接受赋形剂处理的动物相比，预先用Wy - 47,663处理的大鼠中，血管紧张素II诱导的饮水反应的起始明显延迟。然而，Wy - 47,663对血管紧张素II注射后30分钟内的总饮水量没有影响。静脉输注Wy - 47,663(2微克/千克/分钟)未能改变中枢给予血管紧张素II的致渴作用。这些数据表明，脑内而非外周循环中的心房利钠因子可能通过调节中枢肾素 - 血管紧张素系统的致渴作用参与细胞外液量的调节。
