Sumy State University, Ministry of Education and Science of Ukraine, Sumy 40007, Ukraine; Institute of Applied Physics, National Academy of Sciences of Ukraine, Sumy 40000, Ukraine.
Sumy State University, Ministry of Education and Science of Ukraine, Sumy 40007, Ukraine.
Ultrason Sonochem. 2018 Apr;42:84-96. doi: 10.1016/j.ultsonch.2017.11.011. Epub 2017 Nov 8.
The samples of hydroxyapatite and carbonate substituted hydroxyapatite (CHA) were obtained under the influence of physical factors, namely ultrasound (US) and microwave (MW) radiations. The results of Fourier transform infrared spectroscopy and X-ray diffraction analysis have proved the formation of the calcium deficient hydroxyapatite and B-type CHA with the Ca/P ratio in the ranges 1.62-1.87. In vitro studies have showed the increased bioactivity of the samples, synthesized under the influence of physical factors as compared to the standard ones. The samples of both groups, synthesized under the influence of 600 W MW, have shown the greatest stability in biological environment. In vivo tests confirm that obtained under US and MW radiations hydroxyapatite-based biomaterials are biocompatible, non-toxic and exhibit osteoconductive properties. The usage of US and MW radiations can significantly shorten the time (up to 5-20 min) of obtaining of calcium deficient hydroxyapatite and B-type CHA in nanopowder form, close in structure and composition to the biological hydroxyapatite.
在物理因素（即超声（US）和微波（MW）辐射）的影响下，获得了羟基磷灰石和碳酸取代羟基磷灰石（CHA）的样品。傅里叶变换红外光谱和 X 射线衍射分析的结果证明了形成了钙缺乏羟基磷灰石和 B 型 CHA，Ca/P 比在 1.62-1.87 范围内。体外研究表明，与标准样品相比，在物理因素影响下合成的样品具有更高的生物活性。在生物环境中，在 600 W MW 影响下合成的两组样品均表现出最大的稳定性。体内试验证实，在 US 和 MW 辐射下获得的基于羟基磷灰石的生物材料是生物相容的、无毒的，并表现出骨诱导特性。使用 US 和 MW 辐射可以显著缩短获得纳米级钙缺乏羟基磷灰石和 B 型 CHA 的时间（最多 5-20 分钟），其结构和组成与生物羟基磷灰石接近。
