Sukhodub L B, Sukhodub L F, Kumeda M O, Prylutskyy Yu I, Pogorielov M V, Evstigneev M P, Kostjukov V V, Strutynska N Y, Vovchenko L L, Khrapatiy S V, Ritter U
Sumy State University, Sumy, Ukraine.
Taras Shevchenko National University of Kyiv, Kiev, Ukraine.
Prog Biomater. 2020 Jun;9(1-2):1-14. doi: 10.1007/s40204-020-00127-2. Epub 2020 Jan 30.
Single-walled carbon nanotubes (SWCNTs) containing biomaterial with enhanced mechanical properties for the potential orthopedic application were synthesized and investigated. X-ray diffraction and X-ray fluorescence analysis were indications of the formation of calcium-deficient (Ca/P = 1.65) hydroxyapatite (HA) with a small carbonate content under influence of microwave irradiation. The investigated mechanical properties (maximal relative deformation, compressive strength and Young's modulus) of SWCNT loaded HA-alginate composites confirm their dependence on SWCNTs content. The compressive strength of HA-alginate-SWCNT and the HA-alginate control (202 and 159 MPa, respectively) lies within the values characteristic for the cortical bone. The addition of 0.5% SWCNT, in relation to the content of HA, increases the Young's modulus of the HA-alginate-SWCNT (645 MPa) compared to the SWCNT-free HA-alginate sample (563 MPa), and enhances the material shape stability in simulated physiological conditions. Structural modeling of HA-alginate-SWCNT system showed, that physical adsorption of SWCNT into HA-alginate occurs by forming triple complexes stabilized by solvophobic/van der Waals interactions and H-bonds. The high-performance liquid chromatography demonstrated the influence of SWCNTs on the sustained anaesthesinum drug (used as a model drug) release (456 h against 408 h for SWCNT-free sample). Cell culture assay confirmed biocompatibility and stimulation of osteoblast proliferation of 0.05% and 0.5% SWCNT-containing composites during a 3-day cultivation. All these facts may suggest the potential possibility of using the SWCNT-containing materials, based on HA and alginate, for bone tissue engineering.
合成并研究了含有生物材料的单壁碳纳米管（SWCNT），其具有增强的机械性能，可用于潜在的骨科应用。X射线衍射和X射线荧光分析表明，在微波辐射的影响下形成了缺钙（Ca/P = 1.65）且碳酸盐含量低的羟基磷灰石（HA）。对负载SWCNT的HA-藻酸盐复合材料的机械性能（最大相对变形、抗压强度和杨氏模量）进行了研究，证实了它们对SWCNT含量的依赖性。HA-藻酸盐-SWCNT和HA-藻酸盐对照的抗压强度（分别为202和159 MPa）处于皮质骨的特征值范围内。相对于HA的含量，添加0.5%的SWCNT可使HA-藻酸盐-SWCNT的杨氏模量（645 MPa）比不含SWCNT的HA-藻酸盐样品（563 MPa）有所增加，并提高了材料在模拟生理条件下的形状稳定性。HA-藻酸盐-SWCNT系统结构模型表明，SWCNT通过形成由疏溶剂/范德华相互作用和氢键稳定的三元复合物，物理吸附到HA-藻酸盐中。高效液相色谱法表明SWCNT对持续麻醉药物（用作模型药物）释放的影响（含SWCNT样品为456小时，不含SWCNT样品为408小时）。细胞培养试验证实了含0.05%和0.5% SWCNT的复合材料在3天培养过程中的生物相容性以及对成骨细胞增殖的刺激作用。所有这些事实可能表明，基于HA和藻酸盐的含SWCNT材料用于骨组织工程具有潜在可能性。
