Biomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, France.
Tampere University, Faculty of Medicine and Health Technology, Laboratory of Biomaterials and Tissue Engineering, Korkeakoulunkatu 3, 33720 Tampere, Finland.
Biomolecules. 2021 Mar 17;11(3):444. doi: 10.3390/biom11030444.
Hybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two glass compositions (13-93 and 13-93B20 (where 20% of the SiO was replaced with BO)) were introduced in the gelatin matrix. The C (gelatin/GPTMS molar ratio) was kept constant at 500. The hybrids obtained were found to be stable at 37 °C in solution, the condition in which pure gelatin is liquid. All hybrids were characterized by in vitro dissolution in Tris(hydroxymethyl)aminomethane (TRIS) solution (for up to 4 weeks) and Simulated Body Fluid (SBF) (for up to 2 weeks). Samples processed with 13-93B20 exhibited faster initial dissolution and significantly faster precipitation of a hydroxyapatite (HA) layer. The faster ion release and HA precipitation recorded from the G*/13-93B20 samples are attributable to the higher reactivity of borosilicate compared to silicate glass. The MC3T3-E1 cell behavior in direct contact with the hybrids was investigated, showing that the cells were able to proliferate and spread on the developed biomaterials. Tailoring the glass composition allows us to better control the material's dissolution, biodegradability, and bioactivity. Bioactive (especially with 13-93B20 BAG) and biocompatible, the hybrids are promising for bone application.
基于生物活性玻璃 (BAG) 颗粒 (<38 µm) 的杂化支架，通过 3-缩水甘油丙基三甲氧基硅烷 (GPTMS) 与明胶 (G*) 共价连接，已被用于骨生物工程研究。在这项研究中，两种玻璃成分 (13-93 和 13-93B20(其中 20%的 SiO 被 BO 取代)) 被引入明胶基质中。C(明胶/GPTMS 的摩尔比)保持在 500。在 37°C 的溶液中，即纯明胶呈液态的条件下，发现所得的杂化物是稳定的。所有的杂化物都通过在三羟甲基氨基甲烷 (TRIS) 溶液中的体外溶解(长达 4 周)和模拟体液 (SBF)(长达 2 周)进行了表征。用 13-93B20 处理的样品表现出更快的初始溶解和更快的羟基磷灰石 (HA) 层沉淀。与硅酸盐玻璃相比，硼硅玻璃具有更高的反应性，这使得从 G*/13-93B20 样品中记录到更快的离子释放和 HA 沉淀。MC3T3-E1 细胞与杂化物直接接触的行为研究表明，细胞能够在开发的生物材料上增殖和扩散。通过调整玻璃成分，可以更好地控制材料的溶解、生物降解性和生物活性。杂化物具有生物活性(特别是 13-93B20 BAG)和生物相容性，有望应用于骨骼。
