Sato T, Shichishima T, Kimura H, Uchida T, Kariyone S, Ohto H, Maeda H
Scand J Haematol. 1986 May;36(5):415-23. doi: 10.1111/j.1600-0609.1986.tb02275.x.
We identified bone marrow megakaryocytes by an immunocytochemical technique using a monoclonal antibody (TP80) against platelet glycoprotein IIb-IIIa (GPIIb-IIIa). The immunocytochemical technique using TP80, specific to megakaryocytes, enabled us to observe cellular morphology and immunological reaction under light microscopy, and permitted quantitative assessment of megakaryocytes. In normal marrow, TP80 labelled 22 +/- 5 megakaryocytes/10(4) mononuclear cells (mean +/- 1 SD, n = 14). In addition to typical large megakaryocytes, small immature megakaryocytes (less than or equal to 20 micron) were recognized in 10-15% of total megakaryocytes. 4 out of 18 patients with acute myeloblastic leukaemia and 9 of 10 patients with myelodysplastic syndrome showed increased numbers of megakaryocytes. In these patients, cell size distribution was abnormal, i.e., most of the megakaryocytes consisted of small, atypical megakaryocytes. None of the patients with acute lymphoblastic leukaemia showed increased megakaryocytes. Immunocytochemical identification of megakaryocytes using a specific antibody is useful to quantitate the megakaryocytes and to detect the proliferation of atypical megakaryocytes in several leukaemic conditions.
我们使用一种针对血小板糖蛋白IIb-IIIa（GPIIb-IIIa）的单克隆抗体（TP80），通过免疫细胞化学技术鉴定骨髓巨核细胞。使用对巨核细胞特异的TP80的免疫细胞化学技术，使我们能够在光学显微镜下观察细胞形态和免疫反应，并对巨核细胞进行定量评估。在正常骨髓中，TP80标记22±5个巨核细胞/10⁴个单核细胞（平均值±1标准差，n = 14）。除了典型的大巨核细胞外，在总巨核细胞的10% - 15%中可识别出小的未成熟巨核细胞（≤20微米）。18例急性髓细胞白血病患者中有4例，10例骨髓增生异常综合征患者中有9例巨核细胞数量增加。在这些患者中，细胞大小分布异常，即大多数巨核细胞由小的、非典型巨核细胞组成。急性淋巴细胞白血病患者均未出现巨核细胞增多。使用特异性抗体对巨核细胞进行免疫细胞化学鉴定，有助于对巨核细胞进行定量，并在几种白血病情况下检测非典型巨核细胞的增殖。
