Chandrasekaran Rajkuberan, Seetharaman Prabukumar, Krishnan Muthukumar, Gnanasekar Sathishkumar, Sivaperumal Sivaramakrishnan
1Department of Biotechnology, School of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu India.
Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu India.
3 Biotech. 2018 Feb;8(2):83. doi: 10.1007/s13205-018-1105-6. Epub 2018 Jan 16.
This study manifests the larvicidal efficacy of latex extract and silver nanoparticles (CPAgNPs) synthesized using latex, against developing immature juveniles of and . Briefly, the latex was collected and fractioned with different solvents such as chloroform, methanol and aqueously. The obtained crude extracts were subjected to larvicidal activity in the dose-dependent method. After 24 h, the mortality rate was calculated and statistically analyzed. From the results, it was demonstrated that the chloroform extract displayed prominent activity in IInd and IIIrd instar larvae of and with better LC values followed by methanol and aqueous extract. Subsequently, we profiled the qualitative analysis of a chloroform extract through biochemical tests; Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. Moreover, we authenticated the major secondary metabolites and activated larvicidal compound present in the extract. Further, we synthesized CPAgNPs using aqueous latex extract and challenged with IInd and IIIrd instar larvae of and Noticeably, the synthesized nanoproducts were showed 100% mortality in a 24-h treatment with significant LC values. Hence, this study has opened up new vistas in the field of parasitological research to develop latex as a new stratagem in the insect vector management program.
本研究表明了乳胶提取物以及使用乳胶合成的银纳米颗粒（CPAgNPs）对和发育中的未成熟幼虫的杀幼虫效果。简要来说，收集乳胶并用不同溶剂如氯仿、甲醇和水进行分离。将得到的粗提物采用剂量依赖法进行杀幼虫活性测试。24小时后，计算死亡率并进行统计分析。结果表明，氯仿提取物在和的第二龄和第三龄幼虫中表现出显著活性，其LC值优于甲醇提取物和水提取物。随后，我们通过生化测试、傅里叶变换红外光谱和气相色谱 - 质谱联用对氯仿提取物进行了定性分析。此外，我们鉴定了提取物中主要的次生代谢产物和具有杀幼虫活性的化合物。进一步地，我们使用乳胶水提取物合成了CPAgNPs，并对和的第二龄和第三龄幼虫进行测试。值得注意的是，合成的纳米产品在24小时处理中显示出100%的死亡率，且LC值显著。因此，本研究在寄生虫学研究领域开辟了新的前景，将乳胶开发为昆虫媒介管理计划中的一种新策略。
