Centro de Investigação em Biodiversidade e Recursos Genéticos, CIBIO/InBIO, Vairão, Portugal.
Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Porto, Portugal.
Transbound Emerg Dis. 2018 Aug;65(4):983-992. doi: 10.1111/tbed.12830. Epub 2018 Feb 11.
Rabbit haemorrhagic disease (RHD) is a viral disease that affects the European rabbit. RHD was detected in 1984 in China and rapidly disseminated worldwide causing a severe decline in wild rabbit populations. The aetiological agent, rabbit haemorrhagic disease virus (RHDV), is an RNA virus of the family Caliciviridae, genus Lagovirus. Pathogenic (G1-G6 or variants GI.1a-GI.1d) and non-pathogenic strains (GI.4) have been characterized. In 2010, a new variant of RHDV, RHDV2/RHDVb/GI.2, was detected in France. GI.2 arrived to the Iberian Peninsula in 2011, and several recombination events were reported. Here, we sequenced full genomes of 19 samples collected in Portugal between 2014 and 2016. New GI.2 recombinant strains were detected, including triple recombinants. These recombinants possess a non-structural protein p16 related to a non-pathogenic strain. Evolutionary analyses were conducted on GI.2 VP60 sequences. Estimated time to the most recent common ancestor (tMRCA) suggests an emergence of GI.2 in July 2008, not distant from its first detection in 2010. This is the first study on GI.2 evolution and highlights the need of continued monitoring and characterization of complete genome sequences when studying lagoviruses' evolution.
兔出血症（RHD）是一种影响欧洲兔的病毒性疾病。RHD 于 1984 年在中国被发现，并迅速在全球传播，导致野生兔群数量严重下降。病原体是兔出血症病毒（RHDV），它是一种属于杯状病毒科、兔杯状病毒属的 RNA 病毒。已鉴定出致病性（G1-G6 或变体 GI.1a-GI.1d）和非致病性株（GI.4）。2010 年，在法国发现了一种新的 RHDV 变体 RHDV2/RHDVb/GI.2。2011 年，GI.2 到达伊比利亚半岛，并报告了几起重组事件。在此，我们对 2014 年至 2016 年期间在葡萄牙采集的 19 个样本进行了全基因组测序。检测到了新的 GI.2 重组株，包括三重重组株。这些重组株具有与非致病性株相关的非结构蛋白 p16。对 GI.2 VP60 序列进行了进化分析。GI.2 的估计最近共同祖先（tMRCA）时间表明，它于 2008 年 7 月出现，与 2010 年首次发现的时间并不遥远。这是关于 GI.2 进化的首次研究，强调了在研究杯状病毒进化时，需要持续监测和描述完整基因组序列。
