Division of Tissue Engineering and Biophotonics, Dental Institute, King's College London, Guy's Hospital, London, SE1 9RT, United Kingdom.
J Biomed Mater Res A. 2018 Jun;106(6):1575-1584. doi: 10.1002/jbm.a.36367. Epub 2018 Feb 19.
The requirement for improved modeling of cells in culture for study of cell-to-cell interactions has led to an increased focus on three-dimensional (3D) in vitro models. In this study, NG108-15 neural cells and rat sciatic nerve Schwann cells were successfully grown as monocultures and as a coculture on the biocompatible nylon mesh substrates. This has allowed proliferation and interactions between the two cell types to be monitored on the mesh over time. Electrophysiological recordings validated the NG108-15 neural cell differentiation which confirmed the similarity of recorded action potentials in two-dimensional culture environments. A variety of assays have been used, demonstrating the use of nylon as an appropriate substrate for neural cell in vitro studies, and nylon can easily be imaged using inverted microscopy. These studies confirm the suitability of the nylon 3D culture presented for modeling neural cell behavior. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1575-1584, 2018.
为了提高细胞培养模型中细胞间相互作用的模拟效果，人们越来越关注三维（3D）体外模型。在这项研究中，NG108-15 神经细胞和大鼠坐骨神经施万细胞成功地在生物相容性尼龙网基底上进行了单一培养和共培养。这使得两种细胞类型之间的增殖和相互作用可以在网片上随时间进行监测。电生理记录验证了 NG108-15 神经细胞的分化，这证实了在二维培养环境中记录到的动作电位的相似性。已经使用了各种检测方法，证明了尼龙作为体外神经细胞研究的合适基底的用途，并且尼龙可以使用倒置显微镜轻松成像。这些研究证实了所提出的尼龙 3D 培养方法用于模拟神经细胞行为的适用性。© 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1575-1584, 2018.
