MDRL Laboratory, Department of Biochemistry, Ziauudin University, 4/B Shahra e Ghalib, Clifton-6, Karachi, Pakistan.
Int J Infect Dis. 2018 Apr;69:115-119. doi: 10.1016/j.ijid.2018.02.003. Epub 2018 Feb 9.
To find out the association between Human Papilloma Virus (HPV) genotypes 16/18 in Pakistani patients with oral squamous cell carcinoma (OSCC).
DNA from oral rinse of 300 subjects was taken. The subjects included 100 cases with OSCC and 200 controls. Samples were analyzed by both conventional and real time PCR using "HPV consensus Gp5+/Gp6+ and HPV 16, 18 specific primers".
Out of 300 persons, 74/300 (25%) were found to be infected with HPV: "46/100(46%) from cases and 74/200(14%) from controls". The distribution was: HPV16, 6/300 (8%): 4/100 (9%) from OSCC group and 2/200 (8%) from controls while HPV 18 was 9/300(12%): 5/100(11%) from cases and 4/200(16%) from controls. Out of 300 subjects, 26(35%) were infected by "both HPV 16/18 (23(50%) from cases and 3(12%) from controls". Persons who were infected with HPV 16&18 had higher chances to develop OSCC as compared to those who didn't have HPV 16/18 (AOR: 21.4, 95% CI: 5.73 - 80.8).
The exposure to high risk strains of Human papilloma virus (16/18) in combination can be fabricotor of trouble (p<0.001, Adjusted odds ratio; 21.42) in OSCC.
探讨 HPV 基因型 16/18 在巴基斯坦口腔鳞状细胞癌（OSCC）患者中的相关性。
采集 300 例受试者的口腔冲洗液 DNA。受试者包括 100 例 OSCC 患者和 200 例对照。采用常规和实时 PCR 法，用“HPV 通用 Gp5+/Gp6+和 HPV 16、18 特异性引物”对样本进行分析。
在 300 人中，有 74/300（25%）感染了 HPV：“46/100（46%）来自病例，74/200（14%）来自对照”。分布情况为：HPV16，6/300（8%）：4/100（9%）来自 OSCC 组，2/200（8%）来自对照组；HPV18 为 9/300（12%）：5/100（11%）来自病例，4/200（16%）来自对照组。在 300 名受试者中，有 26 名（35%）同时感染了 HPV 16/18（23 名（50%）来自病例，3 名（12%）来自对照）。与未感染 HPV 16/18 的患者相比，感染 HPV 16&18 的患者发生 OSCC 的几率更高（AOR：21.4，95%CI：5.73-80.8）。
高危型 HPV（16/18）联合暴露可能是 OSCC 的致病因素（p<0.001，调整后的比值比；21.42）。
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