Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom 73170, Thailand.
Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
Biosensors (Basel). 2022 May 2;12(5):290. doi: 10.3390/bios12050290.
Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. Hence, we developed a multianalyte electrochemical DNA biosensor that could be used for both oral and cervical samples to detect the high-risk HPV genotypes 16 and 18. The assay involves the sandwich hybridization of the HPV target to the silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with a detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM and 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR/gel electrophoresis detection. The biosensor assay could be completed within 90 min. Due to its simplicity, rapidity, and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories as well as for epidemiological studies.
高危型人乳头瘤病毒（HPV）感染是口腔癌和宫颈癌的主要危险因素。因此，我们开发了一种多分析物电化学 DNA 生物传感器，可用于口腔和宫颈样本检测高危 HPV 基因型 16 和 18。该测定法涉及 HPV 靶标与硅基氧化还原染料报告探针和捕获探针的夹心杂交，然后进行电化学检测。该传感器具有高度的特异性和灵敏度，对 HPV-16 的检测限为 22 fM，对 HPV-18 的检测限为 20 fM，检测范围为 1 fM 至 1 µM。对口腔和宫颈样本的评估表明，生物传感器的结果与巢式 PCR/凝胶电泳检测一致。生物传感器检测可在 90 分钟内完成。由于其简单、快速和高灵敏度，该生物传感器可作为 HPV 在临床实验室以及流行病学研究中的替代检测方法。
