Unité des Maladies à potentiel épidémiques, Maladies émergentes et Zoonoses, Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso.
Infectious Diseases Research Unit, Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.
Int J Infect Dis. 2018 May;70:15-19. doi: 10.1016/j.ijid.2018.02.004. Epub 2018 Feb 9.
In this study, we monitored the seroprevalence of HBV-HDV co-infection in different population groups in the Western part of Burkina Faso, and described the genetic diversity of the detected virus strains.
Between October 2013 and December 2014, venous blood samples were collected from different cohorts (blood donors, pregnant women, outpatients) in the western region of Burkina Faso. Samples were tested for HBsAg and total anti-HDV antibodies. Positive samples were further analysed for HBV-DNA and HDV-RNA. Genotyping of the detected virus strains was done by nucleotide sequencing and phylogenetic analyses.
A total of 841 participants were included in this study. The mean age was 27.45 years (range: 7-89 years). HBsAg was found in 117 (13.9%) participants. Of the HBsAg positive samples, 4 (3.4%) were positive for total anti-HDV antibodies and negative for HDV RNA. Phylogenetic analyses based on the HBV complete genome (n=10) and S fragment sequences (n=35) showed that all strains belonged to genotype E.
Our study showed a high HBsAg prevalence, but a low rate of HDV co-infection in HBsAg carriers from western Burkina Faso. The predominance of HBV genotype E in the country was confirmed. Our findings contribute to a better understanding of the burden of HBV and HDV infection in western Burkina Faso.
本研究旨在监测布基纳法索西部不同人群中乙型肝炎病毒-丁型肝炎病毒（HBV-HDV）合并感染的血清流行率，并描述所检测病毒株的遗传多样性。
2013 年 10 月至 2014 年 12 月，从布基纳法索西部的不同队列（献血者、孕妇、门诊患者）采集静脉血样本。样本检测乙型肝炎表面抗原（HBsAg）和总抗丁型肝炎病毒抗体。阳性样本进一步检测 HBV-DNA 和 HDV-RNA。通过核苷酸测序和系统进化分析对检测到的病毒株进行基因分型。
本研究共纳入 841 名参与者。平均年龄为 27.45 岁（范围：7-89 岁）。117 名（13.9%）参与者 HBsAg 阳性。在 HBsAg 阳性样本中，有 4 名（3.4%）总抗丁型肝炎病毒抗体阳性但 HDV RNA 阴性。基于 HBV 全基因组（n=10）和 S 片段序列（n=35）的系统进化分析显示，所有毒株均属于基因型 E。
本研究显示，布基纳法索西部 HBsAg 阳性者的 HBsAg 流行率较高，但 HDV 合并感染率较低。该结果进一步证实了该国 HBV 基因型 E 的优势地位。本研究结果有助于更好地了解布基纳法索西部 HBV 和 HDV 感染的负担。
