School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Food Res Int. 2018 Mar;105:996-1002. doi: 10.1016/j.foodres.2017.12.009. Epub 2017 Dec 6.
DNA damage and inflammation are promising targets in disease prevention studies. Since these pathways have shown to be modulated by dietary components, investigating the molecular effects of food becomes relevant. This study aimed at investigating the protective effects of cocoplum (Chrysobalanus icaco L.) against doxorubicin (DXR)-induced damage. Rats were treated with cocoplum (100, 200 or 400mg/kg/day) for 14days, associated or not with DXR (15mg/kg b.w.). Tissue-targeted comet assay and the oxidative stress parameters oxidized/reduced glutathione and catalase were investigated in liver, kidney, and heart. The expressions of DNA damage/repair (Gadd45a, Parp1, Xrcc2) and proinflammatory genes (Il-1β, Il-6, Nf-κb, Tnf-α) were performed by real-time quantitative PCR. Cocoplum decreased DNA damage and the expressions of Gadd45a, Il-1β, and Tnf-α induced by DXR. These findings demonstrate that cocoplum fruits possess antigenotoxic and anti-inflammatory effects against DXR-induced damage and encourage other in vivo/clinical studies with this fruit.
DNA 损伤和炎症是疾病预防研究中很有前途的靶点。由于这些途径已被证明可以被膳食成分调节，因此研究食物的分子效应变得相关。本研究旨在探讨可可果（Chrysobalanus icaco L.）对阿霉素（DXR）诱导损伤的保护作用。大鼠用可可果（100、200 或 400mg/kg/天）处理 14 天，同时或不与 DXR（15mg/kg b.w.）一起处理。在肝、肾和心脏中检测组织靶向彗星试验和氧化应激参数氧化/还原型谷胱甘肽和过氧化氢酶。通过实时定量 PCR 检测 DNA 损伤/修复（Gadd45a、Parp1、Xrcc2）和促炎基因（Il-1β、Il-6、Nf-κb、Tnf-α）的表达。可可果降低了 DXR 诱导的 DNA 损伤和 Gadd45a、Il-1β 和 Tnf-α 的表达。这些发现表明可可果具有抗 DXR 诱导损伤的抗原毒性和抗炎作用，并鼓励对这种水果进行其他体内/临床研究。
