a Stem Cell Technology Research Center , Tehran , Iran.
b Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine , Zanjan University of Medical Sciences , Zanjan , Iran.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):733-739. doi: 10.1080/21691401.2018.1434663. Epub 2018 Feb 12.
Transplantation of islet is a promising method in treatment of patients with type 1 diabetes mellitus (T1DM), however, is limited by islet shortage. The aim of this study was to prepare a polyethersulfone (PES) nanofibrous scaffolds to evaluate the pancreatic differentiation of human induced pluripotent stem cells (hiPSCs). The differentiation process in tissue culture dishes and PES scaffolds was evaluated at mRNA and protein level by RT-qPCR and immunofluorescence assay, respectively. The functionality of differentiated cells was determined by insulin and C-peptide release in response to glucose challenges. The results of this study showed that cells cultured on PES nanofibrous scaffolds exhibit more pancreatic β-cell characteristics as they express more pancreatic tissue-specific genes and proteins. Furthermore, the immunoassay showed that differentiated cells in both culture plates and PES scaffolds groups are functional and secrete C-peptide and insulin in response to glucose challenges. Altogether, the results of this study demonstrated that PES nanofibrous scaffold could provide the microenvironment that promotes the differentiation of induced pluripotent stem cells (iPSCs) into insulin producing cells.
胰岛移植是治疗 1 型糖尿病（T1DM）患者的一种很有前途的方法，然而，它受到胰岛短缺的限制。本研究旨在制备聚醚砜（PES）纳米纤维支架，以评估人诱导多能干细胞（hiPSCs）的胰腺分化。通过 RT-qPCR 和免疫荧光检测分别在 mRNA 和蛋白质水平上评估组织培养皿和 PES 支架中的分化过程。通过胰岛素和 C 肽对葡萄糖刺激的释放来确定分化细胞的功能。本研究结果表明，在 PES 纳米纤维支架上培养的细胞表现出更多的胰腺 β 细胞特征，因为它们表达更多的胰腺组织特异性基因和蛋白质。此外，免疫测定表明，在培养板和 PES 支架组中分化的细胞均具有功能，并在葡萄糖刺激下分泌 C 肽和胰岛素。总之，本研究结果表明，PES 纳米纤维支架可以提供促进诱导多能干细胞（iPSCs）分化为胰岛素分泌细胞的微环境。
