a Stem Cell Technology Research Center , Tehran , Iran.
b Dietary Supplements and Probiotic Research Center , Alborz University of Medical Sciences , Karaj , Iran.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S734-S739. doi: 10.1080/21691401.2018.1508031. Epub 2018 Oct 4.
Given the current conditions of life, one of the problems that the world is facing and is rapidly expanding is diabetes. The existing treatment methods are not responsive to these patients. Today, due to the advent of tissue engineering, cell and stem cell therapies, there are many hopes for treating these patients. In the present study, Polyethersulfone (PES) nanofibers were fabricated by electrospinning and then coated by collagen (PES-collagen), since this protein is abundant at the pancreatic extracellular matrix. After scaffold characterization, pancreatic differentiation potential of human induced pluripotent stem cells (hiPSCs) was investigated when cultured on PES-collagen by RT-qPCR, Immunofluorescence staining and insulin and C-peptide release assays. Pancreatic genes and proteins in cultured iPSCs on PES-collagen were expressed significantly higher than those cultured on culture plate as 2 D control group. Although differentiated cells in both groups are functional and secrete C-peptide and insulin in response to glucose challenges according to the immunoassay result. Considered together, PES-collagen demonstrated that it can be effective during pancreatic differentiation of the stem cells and can also be considered as a promising candidate for use in pancreatic tissue engineering application.
鉴于目前的生活条件，世界正面临并迅速扩大的问题之一是糖尿病。现有的治疗方法对此类患者没有反应。如今，由于组织工程、细胞和干细胞疗法的出现，为治疗这些患者带来了许多希望。在本研究中，通过静电纺丝制备了聚醚砜（PES）纳米纤维，然后用胶原蛋白（PES-胶原蛋白）进行涂层，因为这种蛋白质在胰腺细胞外基质中含量丰富。在对支架进行特性描述后，通过 RT-qPCR、免疫荧光染色和胰岛素和 C 肽释放测定，研究了人诱导多能干细胞（hiPSC）在 PES-胶原蛋白上培养时的胰腺分化潜力。与作为 2D 对照组的培养板上培养的 hiPSCs 相比，在 PES-胶原蛋白上培养的 hiPSCs 中的胰腺基因和蛋白质表达显著更高。尽管根据免疫测定结果，两组分化细胞均具有功能并在响应葡萄糖挑战时分泌 C 肽和胰岛素。综上所述，PES-胶原蛋白表明它可以在干细胞的胰腺分化过程中发挥作用，也可以被认为是用于胰腺组织工程应用的有前途的候选物。
