Kobayashi K, Suko M, Yoshida T, Cohen S
Cell Immunol. 1986 Feb;97(2):325-34. doi: 10.1016/0008-8749(86)90403-x.
Doubly immunized guinea pigs may be desensitized with respect to delayed hypersensitivity reactions against both antigens (anergy) by injection of large doses of either one. This anergic response therefore has both a specific and nonspecific component. The specific component of desensitization persists longer than the nonspecific one. In the present study, we have explored the mechanism of both antigen-specific and antigen-nonspecific suppression during the later stages of desensitization. Guinea pigs immunized with two antigens, DNP-KLH and DNP-EA, were desensitized with DNP-EA. The lymph node cells obtained from the animals 1 day after desensitization were unable to produce MIF in the presence of either antigen. The cells obtained 3, 5, and 7 days after desensitization were able to generate MIF when stimulated with the non-specific antigen (DNP-KLH), but not with specific antigen (DNP-EA). It was shown that both T- and non-T-cell fractions obtained 1 day after desensitization had the capacity to antigen-nonspecifically suppress MIF production. In contrast, if the cells were obtained 3 or 5 days after desensitization, T cells could inhibit only the antigen-specific production of MIF, while non-T cells were still capable of suppressing antigen-specific and nonspecific MIF production. Interestingly, when these two populations were mixed back again, it was now only suppressive to the specific antigen-induced MIF production. This latter observation indicates that nonspecific suppressor non-T cells may themselves be regulated by suppressor T cells. Furthermore, antigen-specific suppressor T cells were shown to produce soluble factor(s) which inhibited the production of MIF.
经两次免疫的豚鼠，通过注射大剂量的任何一种抗原，可使其对两种抗原的迟发型超敏反应脱敏（无反应性）。因此，这种无反应性反应既有特异性成分，也有非特异性成分。脱敏的特异性成分比非特异性成分持续时间更长。在本研究中，我们探讨了脱敏后期抗原特异性和抗原非特异性抑制的机制。用两种抗原DNP-KLH和DNP-EA免疫的豚鼠，用DNP-EA进行脱敏。脱敏后1天从动物身上获得的淋巴结细胞，在存在任何一种抗原的情况下都无法产生移动抑制因子（MIF）。脱敏后3、5和7天获得的细胞，在用非特异性抗原（DNP-KLH）刺激时能够产生MIF，但用特异性抗原（DNP-EA）刺激时则不能。结果表明，脱敏后1天获得的T细胞和非T细胞部分都有能力非特异性地抑制MIF的产生。相比之下，如果在脱敏后3或5天获得细胞，T细胞只能抑制MIF的抗原特异性产生，而非T细胞仍然能够抑制抗原特异性和非特异性MIF的产生。有趣的是，当这两个群体再次混合时，现在只对特异性抗原诱导的MIF产生有抑制作用。后一观察结果表明，非特异性抑制性非T细胞本身可能受抑制性T细胞的调节。此外，抗原特异性抑制性T细胞被证明能产生抑制MIF产生的可溶性因子。
