Huchet R
Cell Immunol. 1986 Mar;98(1):188-99. doi: 10.1016/0008-8749(86)90279-0.
Keyhole limpet hemocyanin (KLH) given at high dose (4 mg ip) in mice induced a state of unresponsiveness related to the activation of suppressor T cells. An early pathway of suppression is observed within the first 24 hr following KLH injection and is characterized by its cyclophosphamide (CPM) sensitivity and by the specificity of its effector phase, at the level of KLH helper T cells. A late pathway of suppression occurs at Day 3 following KLH injection and is characterized by its CPM resistance and the nonspecificity of its effector phase acting at the B-cell level. Indeed the anti-FLu antibody response to FLu Ovalbumin or thymus-independent antigen FLu LPS were found altered when these antigens were given with TNP KLH. These two pathways of suppression were found to last 8 months. These results suggest that KLH can trigger in an independent manner two pathways of suppression characterized by different CPM sensitivity and different target cells.
给小鼠腹腔注射高剂量（4毫克）的钥孔戚血蓝蛋白（KLH）会诱导一种与抑制性T细胞激活相关的无反应状态。在注射KLH后的头24小时内可观察到早期抑制途径，其特征在于对环磷酰胺（CPM）敏感，且在KLH辅助性T细胞水平上效应阶段具有特异性。晚期抑制途径在注射KLH后的第3天出现，其特征在于对CPM耐药，且在B细胞水平上效应阶段不具有特异性。实际上，当将这些抗原与三硝基苯基（TNP）-KLH一起给予时，发现对流感卵清蛋白或非胸腺依赖性抗原流感脂多糖的抗流感抗体反应发生了改变。发现这两种抑制途径持续8个月。这些结果表明，KLH可以独立触发两种抑制途径，其特征在于不同的CPM敏感性和不同的靶细胞。
