Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 810008, Xining, China.
Sci Rep. 2018 Feb 12;8(1):2817. doi: 10.1038/s41598-018-21269-1.
Pentatricopeptide repeat (PPR) proteins, which are characterized by tandem 30-40 amino acid sequence motifs, constitute of a large gene family in plants. Some PPR proteins have been identified to play important roles in organellar RNA metabolism and organ development in Arabidopsis and rice. However, functions of PPR genes in woody species remain largely unknown. Here, we identified and characterized a total of 626 PPR genes containing PPR motifs in the Populus trichocarpa genome. A comprehensive genome-wide analysis of the poplar PPR gene family was performed, including chromosomal location, phylogenetic relationships and gene duplication. Genome-wide transcriptomic analysis showed that 154 of the PtrPPR genes were induced by biotic and abiotic treatments, including Marssonina brunnea, salicylic acid (SA), methyl jasmonate (MeJA), mechanical wounding, cold and salinity stress. Quantitative RT-PCR analysis further investigated the expression profiles of 11 PtrPPR genes under different stresses. Our results contribute to a comprehensive understanding the roles of PPR proteins and provided an insight for improving the stress tolerance in poplar.
五肽重复（PPR）蛋白的特征是串联的 30-40 个氨基酸序列基序，构成了植物中一个大的基因家族。一些 PPR 蛋白已被鉴定在拟南芥和水稻的细胞器 RNA 代谢和器官发育中发挥重要作用。然而，木质物种中 PPR 基因的功能在很大程度上仍然未知。在这里，我们在毛白杨基因组中鉴定并鉴定了总共 626 个含有 PPR 基序的 PPR 基因。对杨树 PPR 基因家族进行了全面的全基因组分析，包括染色体定位、系统发育关系和基因复制。全基因组转录组分析表明，154 个 PtrPPR 基因受到生物和非生物处理的诱导，包括杨树叶斑病菌、水杨酸（SA）、茉莉酸甲酯（MeJA）、机械损伤、冷和盐胁迫。定量 RT-PCR 分析进一步研究了 11 个 PtrPPR 基因在不同胁迫下的表达谱。我们的研究结果有助于全面了解 PPR 蛋白的作用，并为提高杨树的抗逆性提供了深入了解。
