Wang Yong, Tan Bao-Cai
Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.
Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China.
Plant Commun. 2025 Feb 10;6(2):101203. doi: 10.1016/j.xplc.2024.101203. Epub 2024 Dec 5.
Pentatricopeptide repeat (PPR) proteins are involved in nearly all aspects of post-transcriptional processing in plant mitochondria and plastids, playing vital roles in plant growth, development, cytoplasmic male sterility restoration, and responses to biotic and abiotic stresses. Over the last three decades, significant advances have been made in understanding the functions of PPR proteins and the primary mechanisms through which they mediate post-transcriptional processing. This review aims to summarize these advancements, highlighting the mechanisms by which PPR proteins facilitate RNA editing, intron splicing, and RNA maturation in the context of organellar gene expression. We also present the latest progress in PPR engineering and discuss its potential as a biotechnological tool. Additionally, we discuss key challenges and questions that remain in PPR research.
五肽重复序列（PPR）蛋白几乎参与植物线粒体和质体转录后加工的各个方面，在植物生长、发育、细胞质雄性不育恢复以及对生物和非生物胁迫的响应中发挥着至关重要的作用。在过去三十年中，我们对PPR蛋白的功能及其介导转录后加工的主要机制的理解取得了重大进展。本综述旨在总结这些进展，重点介绍PPR蛋白在细胞器基因表达背景下促进RNA编辑、内含子剪接和RNA成熟的机制。我们还介绍了PPR工程的最新进展，并讨论了其作为生物技术工具的潜力。此外，我们讨论了PPR研究中仍然存在的关键挑战和问题。
