State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100000, China.
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China.
Int J Mol Sci. 2024 Aug 21;25(16):9048. doi: 10.3390/ijms25169048.
The (Broad-complex, tramtrack, and bric-a-brac) gene family, characterized by a highly conserved BTB domain, is implicated in a spectrum of biological processes, encompassing growth and development, as well as stress responses. Characterization and functional studies of genes in poplar are still limited, especially regarding their response to hormones and biotic/abiotic stresses. In this study, we conducted an HMMER search in conjunction with BLASTp and identified 95 gene models in . Through domain motif and phylogenetic relationship analyses, these proteins were classified into eight families, NPH3, TAZ, Ankyrin, only BTB, BACK, Armadillo, TPR, and MATH. Collinearity analysis of poplar genes with homologs in six other species elucidated evolutionary relationships and functional conservations. RNA-seq analysis of five tissues of poplar identified genes as playing a pivotal role during developmental processes. Comprehensive RT-qPCR analysis of 11 genes across leaves, roots, and xylem tissues revealed their responsive expression patterns under diverse hormonal and biotic/abiotic stress conditions, with varying degrees of regulation observed in the results. This study marks the first in-depth exploration of the gene family in poplar, providing insights into the potential roles of genes in hormonal regulation and response to stress.
(Broad-complex、tramtrack、bric-a-brac) 基因家族，以高度保守的 BTB 结构域为特征，涉及一系列生物学过程，包括生长发育以及应激反应。杨树中 基因的特征和功能研究仍然有限，特别是关于它们对激素和生物/非生物胁迫的反应。在这项研究中，我们进行了 HMMER 搜索，并结合 BLASTp 鉴定了 中的 95 个 基因模型。通过结构域基序和系统发育关系分析，这些蛋白质被分为八个家族：NPH3、TAZ、Ankyrin、仅 BTB、BACK、Armadillo、TPR 和 MATH。杨树 基因与其他六个物种的同源基因的共线性分析阐明了进化关系和功能保守性。杨树五个组织的 RNA-seq 分析确定了 基因在发育过程中起着关键作用。对叶片、根和木质部组织中的 11 个基因进行全面的 RT-qPCR 分析表明，它们在不同的激素和生物/非生物胁迫条件下表现出响应表达模式，结果显示出不同程度的调控。本研究首次深入探讨了杨树中的 基因家族，为 基因在激素调节和应激反应中的潜在作用提供了线索。
