Ojha Swati, Kapoor Sumeet, Mishra Saroj
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz-Khas, New Delhi, 110016 India.
Indian J Microbiol. 2018 Mar;58(1):118-122. doi: 10.1007/s12088-017-0686-9. Epub 2017 Oct 27.
This study reports extraction and characterization of carotenoid pigments from , a non-photosynthetic bacterium, cultivated in Luria-Bertani (LB) medium. The isolate was identified to be moderately halo- and osmo-tolerant capable of withstanding high (~ 6%) salt and sugar (30% w/v sucrose, 20% w/v glucose) concentrations after a brief period of adaptation. The pigments were characterized using a combination of UV-Vis spectral analysis with the λ at 407, 436 and 466 nm and ESI-MS with an value at 536.44. The absorption profile of the pigments and their nature was influenced by carbon, nitrogen source and presence of salt in the growth medium. Highest level of pigment (~ 16 g kg dry wt cells) was produced in NHCl supplemented LB medium. The pigment displayed free radical scavenging, anticancer activity, characteristic of the plant carotenoids. Based on the accumulation of pigments under different conditions, a biochemical pathway for synthesis of neurosporene was proposed.
本研究报告了从一株在Luria-Bertani（LB）培养基中培养的非光合细菌中提取和表征类胡萝卜素色素的过程。经鉴定，该分离株具有中等程度的耐盐和耐渗透压能力，在短暂适应后能够耐受高浓度（约6%）的盐和糖（30% w/v蔗糖，20% w/v葡萄糖）。使用紫外-可见光谱分析（波长分别为407、436和466 nm）和电喷雾电离质谱（质荷比为536.44）相结合的方法对色素进行了表征。色素的吸收光谱及其性质受生长培养基中的碳源、氮源和盐的存在影响。在添加氯化铵的LB培养基中产生的色素水平最高（约16 g/kg干重细胞）。该色素表现出自由基清除、抗癌活性，具有植物类胡萝卜素的特征。基于不同条件下色素的积累情况，提出了神经孢烯合成的生化途径。
