耐紫外线南极微杆菌 LEMMJ01 的类胡萝卜素。
Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01.
机构信息
BIOINOVAR - Biocatalysis, Bioproducts and Bioenergy, Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
LaRBio - Radiations and Biology Laboratory, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
出版信息
Sci Rep. 2019 Jul 2;9(1):9554. doi: 10.1038/s41598-019-45840-6.
The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
从南极土壤中分离到的不动杆菌属 LEMMJ01 不属于 RDP 数据库中鉴定的任何近缘种。与野生型大肠杆菌 K12A15 相比，在紫外辐射（A、B 和 C 波段）下，不动杆菌属细胞的存活分数要高得多。特别是对于一种南极细菌，观察到对高剂量 UV-B 的表达抗性。过夜生长的 DNA 修复能力增强的大肠杆菌中添加 0.1 mg/ml 或 1 mg/ml 由不动杆菌属产生的全色素提取物增加了其对 UV-B 辐射的部分抗性，表明部分抗性似乎与色素的光保护有关。扫描电子显微镜显示，仅在大肠杆菌中，UV-A 和 UV-B 导致了膜的改变。APCI-MS 指纹图谱通过 HPLC-MS/MS 分析首次在该细菌中揭示了神经孢烯（m/z 580、566、522、538 和 524）的诊断离子协同作用。类胡萝卜素在小鼠细胞、人角质形成细胞和成纤维细胞中也没有光毒性和细胞毒性作用。
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