Lv Lin, Huo Ximei, Wen Luhua, Gao Zhihong, Khalil-Ur-Rehman Muhammad
Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2018 Jan 26;9:27. doi: 10.3389/fpls.2018.00027. eCollection 2018.
Bud dormancy release is regulated by gibberellins (GAs). DELLA proteins are highly conserved and act as negative regulators in GA signaling pathway. The present study established a relationship between in Japanese apricot and GA levels during dormancy release of floral buds. Overexpression of in poplar delayed the onset of bud dormancy and resulted in dwarf plants, relative to wild-type trees. exhibited higher expression during ecodormancy and relatively lower expression during endodormancy. The relative level of GA exhibited an increasing trend at the transition from endodormancy to ecodormancy and displayed a similar expression pattern of genes related to GA metabolism, , , in both Japanese apricot and transgenic poplar. These results suggests that acts as an integrator and negative regulator of dormancy via a GA-signaling pathway. Moreover, an interaction between and SLY1 in a yeast two hybrid (Y2H) system further suggests that SCF E3 ubiquitin ligases, such as , may be a critical factor in the regulation of through an SCF -proteasome pathway. Our study demonstrated that plays a negative role in bud dormancy release by regulating the GA biosynthetic enzymes, and and the GA receptor, .
芽休眠解除受赤霉素（GAs）调控。DELLA蛋白高度保守，在GA信号通路中起负调控作用。本研究建立了梅花花芽休眠解除过程中[具体基因名称未给出]与GA水平之间的关系。与野生型杨树相比，杨树中[具体基因名称未给出]的过表达延迟了芽休眠的开始并导致植株矮小。[具体基因名称未给出]在生态休眠期表达较高，而在深休眠期表达相对较低。从深休眠向生态休眠转变时，GA的相对水平呈上升趋势，并且在梅花和转基因杨树中与GA代谢相关的基因[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]表现出相似的表达模式。这些结果表明[具体基因名称未给出]通过GA信号通路作为休眠的整合因子和负调控因子。此外，在酵母双杂交（Y2H）系统中[具体基因名称未给出]与SLY1之间的相互作用进一步表明，SCF E3泛素连接酶，如[具体基因名称未给出]，可能是通过SCF[具体基因名称未给出]-蛋白酶体途径调控[具体基因名称未给出]的关键因子。我们的研究表明，[具体基因名称未给出]通过调节GA生物合成酶[具体基因名称未给出]、[具体基因名称未给出]和GA受体[具体基因名称未给出]在芽休眠解除中起负作用。
