Häkkinen Suvi T, Reuter Lauri, Nuorti Ninni, Joensuu Jussi J, Rischer Heiko, Ritala Anneli
VTT Technical Research Centre of Finland Ltd., Espoo, Finland.
Front Plant Sci. 2018 Jan 26;9:45. doi: 10.3389/fpls.2018.00045. eCollection 2018.
Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein-Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named 'Hulk,' produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43-55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.
随着对无动物产品和生产工艺的需求日益增加，植物细胞成为生产重组蛋白的一个有吸引力的平台。将植物细胞用作生产宿主的挑战之一是昂贵的培养基成分带来的成本。在这项工作中，目标是在不影响生物量积累和重组蛋白产量的前提下，优化营养培养基中最昂贵成分的水平。使用野生型BY-2培养物和表达绿色荧光蛋白-疏水蛋白I（GFP-HFBI）融合蛋白的转基因烟草BY-2来确定最便宜的培养基组成。一个特别高产的BY-2克隆，名为“Hulk”，在悬浮培养中产生了1.1±0.2 g/l的GFP-HFBI，并在长期继代培养过程中保持了其高性能。此外，两种培养物都成功地进行了冷冻保存，从而使这种植物细胞宿主能够真正应用于工业生产。通过优化培养基，生物量成本降低了43-55%，重组蛋白生产成本降低了高达69%。
