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晚期而非早期传代的间充质干细胞对骨关节炎动物模型疼痛行为的治疗益处。

Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis.

作者信息

Chapman Victoria, Markides Hareklea, Sagar Devi Rani, Xu Luting, Burston James J, Mapp Paul, Kay Alasdair, Morris Robert H, Kehoe Oksana, El Haj Alicia J

机构信息

Arthritis Research UK Pain Centre and School of Life Sciences, University of Nottingham, Nottingham, UK.

Institute for Science and Technology in Medicine, Guy Hilton Research Centre, Keele University, Staffordshire, UK.

出版信息

Stem Cells Int. 2017;2017:2905104. doi: 10.1155/2017/2905104. Epub 2017 Dec 24.

DOI:10.1155/2017/2905104
PMID:29434641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5757143/
Abstract

BACKGROUND

Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA.

METHODS

Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5 × 10 late passage MSCs labelled with 10 g/ml SiMAG, 1.5 × 10 late passage mesenchymal stem cells, the steroid Kenalog (200 g/20 L), 1.5 × 10 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint.

RESULTS

Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology.

CONCLUSION

Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.

摘要

背景

间充质干细胞（MSCs）在治疗骨关节炎（OA）关节病变和疼痛方面具有治疗潜力。本研究的目的是确定传代次数对MSCs在OA啮齿动物模型中对疼痛行为以及软骨和骨特征的影响。

方法

大鼠在麻醉下接受内侧半月板横断术（MNX）或假手术。大鼠接受关节内注射以下物质之一：用10μg/ml SiMAG标记的1.5×10晚期传代MSCs、1.5×10晚期传代间充质干细胞、类固醇曲安奈德（200μg/20μL）、1.5×10早期传代MSCs或无血清培养基（SFM）。假手术大鼠接受关节内注射SFM。在模型诱导后第42天之前对疼痛行为进行量化。使用磁共振成像（MRI）在膝关节内定位标记细胞。

结果

晚期传代MSCs和曲安奈德减轻了MNX大鼠已有的疼痛行为，但在研究期末未改变MNX诱导的关节病变。早期传代MSCs在注射后长达一周内加剧了MNX诱导的疼痛行为，且未改变关节病变。

结论

我们的数据首次证明了传代次数在影响MSCs在OA疼痛模型中的治疗效果方面的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/190b37e13cdd/SCI2017-2905104.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/7bb71114b5e4/SCI2017-2905104.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/833d06ac1a9b/SCI2017-2905104.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/bf4b33cedd62/SCI2017-2905104.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/350115d1623a/SCI2017-2905104.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/190b37e13cdd/SCI2017-2905104.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/7bb71114b5e4/SCI2017-2905104.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/833d06ac1a9b/SCI2017-2905104.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/bf4b33cedd62/SCI2017-2905104.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/350115d1623a/SCI2017-2905104.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1df3/5757143/190b37e13cdd/SCI2017-2905104.005.jpg

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