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微小RNA-93通过直接靶向鼻咽癌中的细胞周期蛋白依赖性激酶抑制剂1A来增强细胞增殖。

miR-93 enhances cell proliferation by directly targeting CDKN1A in nasopharyngeal carcinoma.

作者信息

Zhang Yingyao, Xu Zhina

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China.

出版信息

Oncol Lett. 2018 Feb;15(2):1723-1727. doi: 10.3892/ol.2017.7492. Epub 2017 Nov 24.

DOI:10.3892/ol.2017.7492
PMID:29434867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5774441/
Abstract

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy of the head and neck with the highest incidence rate in southern China. The aim of the present study was to understand the molecular mechanisms that underlie the progression of NPC. The relative expression of miR-93 and CDKN1A was detected by the reverse-transcription quantitative PCR. Western blot analysis was applied to detect the protein levels of genes. Luciferase activity report was applied to verify the target of miRNA. Cell growth was assayed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. miR-93 was upregulated in NPC tissues and cell lines compared with normal samples. Re-expression of miR-93 promoted cell growth as determined by the MTT assay. CDKN1A was identified by luciferase reporter as a direct target of miR-93. Its expression was downregulated by miR-93. Furthermore, the results showed that the expression of miR-93 was inversely correlated with the expression of CDKN1A protein. miR-93 enhanced cell proliferation in NPC by directly targeting CDKN1A. It is suggested that miR-93/CDKN1A axis may present a new target for the treatment of NPC.

摘要

鼻咽癌（NPC）是一种头颈部上皮性恶性肿瘤，在中国南方发病率最高。本研究的目的是了解鼻咽癌进展的分子机制。采用逆转录定量PCR检测miR-93和CDKN1A的相对表达。应用蛋白质印迹分析检测基因的蛋白质水平。应用荧光素酶活性报告法验证miRNA的靶标。使用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法检测细胞生长情况。与正常样本相比，miR-93在鼻咽癌组织和细胞系中上调。MTT法检测结果显示，miR-93的重新表达促进了细胞生长。荧光素酶报告基因鉴定CDKN1A为miR-93的直接靶标。miR-93使其表达下调。此外，结果表明miR-93的表达与CDKN1A蛋白的表达呈负相关。miR-93通过直接靶向CDKN1A增强鼻咽癌细胞增殖。提示miR-93/CDKN1A轴可能为鼻咽癌治疗提供新靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/2e77560762a5/ol-15-02-1723-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/765c502593cb/ol-15-02-1723-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/82a03cb9315b/ol-15-02-1723-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/aff61d38ef68/ol-15-02-1723-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/2e77560762a5/ol-15-02-1723-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/765c502593cb/ol-15-02-1723-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/82a03cb9315b/ol-15-02-1723-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/aff61d38ef68/ol-15-02-1723-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c8/5774441/2e77560762a5/ol-15-02-1723-g03.jpg

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