Tam Ka-Wai, Ho Chi-Tang, Tu Shih-Hsin, Lee Wen-Jui, Huang Ching-Shui, Chen Ching-Shyang, Wu Chih-Hsiung, Lee Chia-Hwa, Ho Yuan-Soon
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Department of Surgery, Division of General Surgery, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan.
Oncotarget. 2017 Dec 17;9(4):4593-4606. doi: 10.18632/oncotarget.23390. eCollection 2018 Jan 12.
Vitamin E (Vit. E) is considered an essential dietary nutrient for humans and animals. An enormous body of evidence indicates the biological and protective effects of Vit. E consumption. Tocopherol-associated protein (TAP) is a major tocopherol-binding protein affecting Vit. E stimulation and downstream signaling transduction. However, how Vit. E utilizes TAP as an anti-cancer mechanism remains unclear. Microarray analysis of signature gene profiles in breast cancer cells treated with α-tocopheryl succinate (α-TOS, a Vit. E isoform) resulted in cell cycle arrest and anti-cancer activity in breast cancer cells. Pterostilbene (PS), a natural dietary antioxidant found in blueberries, in combination with α-TOS synergistically maximized breast cancer cell growth inhibition by disrupting signal transduction, transcription factors and cell cycle proteins. In a xenograft mouse model, PS treatment with Vit. E inhibited breast tumor growth and cell invasion, which were evaluated using our recently developed circulating tumor cell (CTC) detection assay. Because dietary Vit. E and PS supplementation contributed to preventative and therapeutic effects and , this combination may benefit breast cancer therapy in the clinic.
维生素E（Vit.E）被认为是人类和动物必需的膳食营养素。大量证据表明摄入Vit.E具有生物学和保护作用。生育酚相关蛋白（TAP）是一种主要的生育酚结合蛋白，影响Vit.E的刺激作用和下游信号转导。然而，Vit.E如何利用TAP作为抗癌机制仍不清楚。对用α-生育酚琥珀酸酯（α-TOS，一种Vit.E异构体）处理的乳腺癌细胞中的特征基因谱进行微阵列分析，导致乳腺癌细胞出现细胞周期停滞和抗癌活性。紫檀芪（PS）是蓝莓中发现的一种天然膳食抗氧化剂，与α-TOS联合使用，通过破坏信号转导、转录因子和细胞周期蛋白，协同最大化抑制乳腺癌细胞生长。在异种移植小鼠模型中，用Vit.E进行PS治疗可抑制乳腺肿瘤生长和细胞侵袭，这是使用我们最近开发的循环肿瘤细胞（CTC）检测方法进行评估的。由于膳食补充Vit.E和PS具有预防和治疗作用，因此这种组合可能对临床乳腺癌治疗有益。
