Janus T J, Braun D P, Harris J E
Clin Immunol Immunopathol. 1986 Oct;41(1):26-34. doi: 10.1016/0090-1229(86)90048-6.
The ability of fibrinogen degradation products (FDP) to influence the regulatory function of adherent cells from peripheral blood mononuclear cells (PBMC) was evaluated. FDP were prepared by digestion of fibrin clots with plasmin. These FDP were incubated overnight with glass-adherent cells following which these treated and untreated cells were cocultivated with fresh autologous responder PBMC in the presence of the T-cell mitogen, phytohemagglutinin (PHA). Lipid metabolism of FDP-treated monocytes was evaluated in cells that had been prelabeled with [3H]arachidonic acid (AA) prior to their overnight incubation with FDP; supernatants were analyzed for conversion of AA to cyclooxygenase and lipoxygenase products by thin-layer chromatography. Treatment of glass-adherent cells with the FDP digests converted these monocytes into suppressor cells. The suppression exerted by these cells in the PHA assay was dose dependent. The suppression exerted by FDP-pretreated monocytes was reversed by treating the PHA-stimulated cocultures with indomethacin and was associated with increased cyclooxygenase activity. These studies demonstrated that FDP can alter T-cell immune function through the induction of monocyte suppressor cells; the means by which that occurs is associated with stimulation of lipid metabolism and secretion of eicosanoids with immunoregulatory capacity.
评估了纤维蛋白原降解产物（FDP）对外周血单个核细胞（PBMC）贴壁细胞调节功能的影响。通过用纤溶酶消化纤维蛋白凝块制备FDP。将这些FDP与玻璃贴壁细胞孵育过夜，之后将这些处理过和未处理的细胞在T细胞丝裂原植物血凝素（PHA）存在下与新鲜的自体反应性PBMC共培养。在用[3H]花生四烯酸（AA）预标记过夜后再与FDP孵育的细胞中评估FDP处理的单核细胞的脂质代谢；通过薄层色谱法分析上清液中AA向环氧化酶和脂氧合酶产物的转化。用FDP消化物处理玻璃贴壁细胞可将这些单核细胞转化为抑制细胞。这些细胞在PHA试验中发挥的抑制作用是剂量依赖性的。用吲哚美辛处理PHA刺激的共培养物可逆转FDP预处理的单核细胞发挥的抑制作用，且这与环氧化酶活性增加有关。这些研究表明，FDP可通过诱导单核细胞抑制细胞来改变T细胞免疫功能；其发生方式与脂质代谢的刺激以及具有免疫调节能力的类花生酸的分泌有关。
