Braum D P, Harris J E, Rubenstein M
J Immunopharmacol. 1984;6(3):227-36. doi: 10.3109/08923978409019463.
The relationship between the conversion of arachidonic acid (AA) to E series prostaglandins (PGE), indomethacin-sensitive immunoregulation and lymphocyte PGE sensitivity was investigated in the peripheral blood mononuclear cells (PBMC) of normal subjects and disseminated solid tumor patients. Production of PGE was assessed by thin layer chromatography of ether-extracted glass adherent cells following a 24-hour pulse with 3H-AA. Immunoregulatory cell function was assessed in PHA-stimulated PBMC cultured in the presence of the prostaglandin synthetase inhibitor, indomethacin. Lymphocyte PGE sensitivity was assessed in PHA stimulated glass nonadherent cells cultured in the presence of 10(-8) M PGE. The cells from cancer patients demonstrated greater AA conversion to PGE and greater indomethacin sensitive immunoregulatory cell function than the cells of normal subjects. However, lymphocyte PGE sensitivity was comparable for both groups. When levels of arachidonic acid conversion to PGE were correlated to levels of indomethacin-sensitive immunoregulatory cell function by linear regression analysis, a significant correlation was found. These data suggest that the increased indomethacin-sensitive immunoregulatory cell function seen in PBMC from cancer patients can be directly correlated with increased production of E series prostaglandins by cancer patient peripheral blood monocytes.
在正常受试者和播散性实体瘤患者的外周血单个核细胞（PBMC）中，研究了花生四烯酸（AA）向E系列前列腺素（PGE）的转化、吲哚美辛敏感的免疫调节与淋巴细胞PGE敏感性之间的关系。在用3H-AA进行24小时脉冲处理后，通过对乙醚提取的玻璃贴壁细胞进行薄层色谱分析来评估PGE的产生。在前列腺素合成酶抑制剂吲哚美辛存在的情况下，对PHA刺激的PBMC进行培养，以评估免疫调节细胞功能。在10（-8）M PGE存在的情况下，对PHA刺激的玻璃非贴壁细胞进行培养，以评估淋巴细胞PGE敏感性。癌症患者的细胞比正常受试者的细胞表现出更高的AA向PGE的转化以及更高的吲哚美辛敏感的免疫调节细胞功能。然而，两组的淋巴细胞PGE敏感性相当。通过线性回归分析将花生四烯酸向PGE的转化水平与吲哚美辛敏感的免疫调节细胞功能水平相关联时，发现了显著的相关性。这些数据表明，在癌症患者PBMC中看到的吲哚美辛敏感的免疫调节细胞功能增加可能与癌症患者外周血单核细胞E系列前列腺素产生增加直接相关。
