Department of Chemistry, Iowa State University, 1605 Gilman Hall, Ames, IA, 50011, USA.
Anal Bioanal Chem. 2018 Jul;410(19):4567-4573. doi: 10.1007/s00216-018-0898-9. Epub 2018 Feb 12.
Ionic liquids are a class of solvents and materials that hold great promise in bioanalytical chemistry. Task-specific ionic liquids have recently been designed for the selective extraction, separation, and detection of proteins, peptides, nucleic acids, and other physiologically relevant analytes from complex biological samples. To facilitate rapid bioanalysis, ionic liquids have been integrated in miniaturized and automated procedures. Bioanalytical separations have also benefited from the modification of nonspecific magnetic materials with ionic liquids or the implementation of ionic liquids with inherent magnetic properties. Furthermore, the direct detection of the extracted molecules in the analytical instrument has been demonstrated with structurally tuned ionic liquids and magnetic ionic liquids, providing a significant advantage in the analysis of low-abundance analytes. This article gives an overview of these advances that involve the application of ionic liquids and derivatives in bioanalysis. Graphical abstract Ionic liquids, magnetic ionic liquids, and ionic liquid-based sorbents are increasing the speed, selectivity, and sensitivity in the analysis of biological samples.
离子液体是一类溶剂和材料，在生物分析化学中具有很大的应用前景。针对特定任务的离子液体最近已被设计用于从复杂的生物样品中选择性提取、分离和检测蛋白质、肽、核酸和其他生理相关分析物。为了促进快速生物分析，离子液体已被整合到小型化和自动化的程序中。生物分析分离也受益于离子液体对非特异性磁性材料的修饰或具有固有磁性的离子液体的实施。此外，通过结构调整的离子液体和磁性离子液体，已经可以在分析仪器中直接检测提取的分子，这在分析低丰度分析物方面具有显著优势。本文概述了这些进展，其中涉及离子液体及其衍生物在生物分析中的应用。
