Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, 608002, India.
Department of Oral & Maxillofacial Pathology, Rajah Muthiah Dental College & Hospital, Annamalai University, Annamalainagar, Tamil Nadu, India.
Mol Cell Biochem. 2018 Nov;448(1-2):145-153. doi: 10.1007/s11010-018-3321-0. Epub 2018 Feb 12.
7,12-Dimethylbenz(a)anthracene (DMBA)-induced hamster buccal pouch carcinogenesis is widely preferred to assess the tumor-inhibiting efficacy of the medicinal plants or their constituents. The present study explores the tumor-inhibiting potential of esculetin by utilizing the status of lipid peroxidation by products (thiobarbituric acid reactive substances), antioxidants (vitamin E, reduced glutathione, superoxide dismutase, catalase, and glutathione peroxidase), and phase I and phase II detoxification agents as biochemical end points and by using histopathological studies as well in DMBA-induced hamster buccal pouch carcinogenesis. Oral tumors developed in the buccal pouch were subjected to histopathological studies, and were confirmed as oral squamous cell carcinoma. Hamsters treated with DMBA alone showed an abnormal pattern of lipid peroxidation, antioxidants, and detoxification agents as compared to control hamsters. The status of the above-mentioned biochemical markers and histopathological abnormalities were found to be reversed in DMBA + esculetin-treated hamsters. The result of the present study thus indicates the tumor preventive potential of esculetin in DMBA-induced oral carcinogenesis.
7,12-二甲基苯并(a)蒽（DMBA）诱导的颊囊癌变在评估药用植物或其成分的肿瘤抑制功效方面被广泛应用。本研究利用脂质过氧化产物（硫代巴比妥酸反应物质）、抗氧化剂（维生素 E、还原型谷胱甘肽、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）以及 I 相和 II 相解毒剂的状态，通过组织病理学研究，探讨了秦皮素对 DMBA 诱导的颊囊癌变的肿瘤抑制潜力。口腔肿瘤在颊囊中发展，并进行了组织病理学研究，被确认为口腔鳞状细胞癌。与对照组相比，单独用 DMBA 处理的仓鼠表现出异常的脂质过氧化、抗氧化剂和解毒剂模式。DMBA+秦皮素处理的仓鼠上述生化标志物和组织病理学异常的状态得到了逆转。因此，本研究结果表明秦皮素具有预防 DMBA 诱导的口腔癌发生的潜力。
