Bastos Natália Marins, Goulart Rodrigo Silva, Bridi Alessandra, Mazzarella Rosane, Alves Luana, da Silva Rosa Paola Maria, de Francisco Strefezzi Ricardo, Paskoski Lindsay Baltel, Nociti Ricardo Perecin, Sangalli Juliano Rodrigues, Saldanha Schaienni Fontoura, de Souza Camila Azzolin, Dos Santos Angélica Camargo, Chiaratti Marcos Roberto, Pugliesi Guilherme, Meirelles Flávio Vieira, Perecin Felipe, da Silveira Juliano Coelho
Department of Veterinary Medicine, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo, São Paulo, Brazil.
PLoS One. 2025 Jun 23;20(6):e0326138. doi: 10.1371/journal.pone.0326138. eCollection 2025.
To analyze the effects of high body energy reserve (BER) within the oviductal environment and its composition, Nellore cows were fed two different nutritional plans to obtain animals with moderate BER (MBER) and high BER (HBER). After obtaining the groups with different BERs, all animals were subjected to oestrus synchronization and artificial insemination, and 120 hours after ovulation induction, the cows were slaughtered, the reproductive tract was removed, and the ipsilateral oviduct to the corpus luteum was collected and dissected. Analyses were performed only for animals that had an 8-cell embryo in the isthmus. After embryo identification, we evaluated the molecular profiles of extracellular vesicles from oviductal flushing (OF-EVs) and luminal epithelial cells (OV-Cell) and performed histomorphological analysis of oviductal tissue from the ampullary and isthmic oviductal regions. The HBER group presented higher concentrations of ampullary extracellular vesicles (AMP-EVs) and larger sizers of isthmic extracellular vesicles (IST-EVs). The miRNA profile of AMP-EVs showed that the differentially expressed miRNAs were predicted to regulate pathways associated with cell growth, migration, differentiation and metabolism, with the HBER group being more susceptible to insulin modulation. The MBER animals showed greater ampullary vascularization than the HBER animals did. Additionally, the miRNA profile and differential gene expression (DEG) data obtained for ampullary (AMP-Cell) and isthmic (IST-Cell) luminal epithelial cells revealed pathways related to insulin metabolism. Thus, elevated BER may lead to oviductal insulin resistance, affecting normal functioning and, probably, embryo metabolism during early development, thus impacting gestational rates in these animals.
为了分析输卵管环境中高机体能量储备（BER）及其组成的影响，对内洛尔牛饲喂两种不同的营养方案，以获得具有中等BER（MBER）和高BER（HBER）的动物。在获得具有不同BER的组后，所有动物都进行发情同步和人工授精，在诱导排卵120小时后，宰杀母牛，取出生殖道，收集并解剖与黄体同侧的输卵管。仅对在峡部有8细胞胚胎的动物进行分析。在鉴定胚胎后，我们评估了输卵管冲洗液细胞外囊泡（OF-EVs）和腔上皮细胞（OV-Cell）的分子谱，并对输卵管壶腹部和峡部区域的组织进行了组织形态学分析。HBER组的壶腹部细胞外囊泡（AMP-EVs）浓度较高，峡部细胞外囊泡（IST-EVs）尺寸较大。AMP-EVs的miRNA谱显示，差异表达的miRNA预计可调节与细胞生长、迁移、分化和代谢相关的途径，HBER组对胰岛素调节更敏感。MBER动物的壶腹部血管化程度高于HBER动物。此外，从壶腹部（AMP-Cell）和峡部（IST-Cell）腔上皮细胞获得的miRNA谱和差异基因表达（DEG）数据揭示了与胰岛素代谢相关的途径。因此，BER升高可能导致输卵管胰岛素抵抗，影响早期发育过程中的正常功能，可能还会影响胚胎代谢，从而影响这些动物的妊娠率。
