Division of Surgical Pathology, Tokyo Medical and Dental University Hospital, Tokyo, Japan.
Department of Diagnostic Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Histopathology. 2018 Jun;72(7):1199-1208. doi: 10.1111/his.13486. Epub 2018 Mar 25.
A number of homeobox transcriptional factors are utilised as organ-specific markers in the histopathological diagnosis of neoplasms. We have screened a homeobox gene that is expressed specifically in normal sweat gland cells and is useful for the histopathological diagnosis of sweat gland neoplasms.
By screening an open database resource of The Human Protein Atlas, 37 genes among the 235 homeobox transcriptional factors were found to be expressed specifically in the skin. Among those 37 genes, the engrailed homeobox 1 (En1) was expressed in normal eccrine glands but not in the epidermal keratinocytes. Expression of En1 was found throughout the eccrine glands, but not in the apocrine secretory coils, sebaceous glands or hair follicles. Expression of En1 was examined immunohistochemically in 111 cases of cutaneous epithelial neoplasms. All nine cases of poroma, seven cases of spiradenoma and six cases of syringoma, which are considered to differentiate towards eccrine glands, showed positive nuclear staining in most of the tumour cells. Sebaceous gland and hair follicle tumours were immunonegative. En1 was expressed focally in the epidermal neoplasms of seborrheic keratosis and squamous cell carcinoma.
Engrailed homeobox 1 was expressed specifically in normal eccrine glands and was expressed in most of the tumour cells of sweat gland neoplasms with eccrine gland differentiation. En1 was expressed focally in epidermal neoplasms; however, it was absent in sebaceous or hair follicle neoplasms. These findings will help in the histopathological diagnosis as well as understanding of the histogenesis of sweat gland neoplasms.
许多同源盒转录因子被用作肿瘤组织病理学诊断中的器官特异性标志物。我们筛选了一种在正常汗腺细胞中特异性表达的同源盒基因，该基因可用于汗腺肿瘤的组织病理学诊断。
通过筛选人类蛋白质图谱的开放数据库资源，在 235 种同源盒转录因子中发现 37 种基因特异性表达于皮肤。在这 37 种基因中， engrailed homeobox 1（En1）在正常的小汗腺中表达，但不在表皮角质形成细胞中表达。En1 的表达贯穿整个小汗腺，但不在大汗腺分泌管、皮脂腺或毛囊中表达。我们用免疫组织化学方法检测了 111 例皮肤上皮肿瘤病例中 En1 的表达。所有 9 例汗孔瘤、7 例小汗腺汗腺瘤和 6 例汗管瘤，被认为向小汗腺分化，其大多数肿瘤细胞均呈阳性核染色。皮脂腺和毛囊肿瘤免疫阴性。En1 在脂溢性角化病和鳞状细胞癌的表皮肿瘤中呈局灶性表达。
engrailed homeobox 1 特异性表达于正常小汗腺，并且在具有小汗腺分化的汗腺肿瘤的大多数肿瘤细胞中表达。En1 在表皮肿瘤中呈局灶性表达，但在皮脂腺或毛囊肿瘤中不存在。这些发现将有助于组织病理学诊断以及对汗腺肿瘤发生机制的理解。
