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利用皮肤表面脂质中的 mRNA 进行无创性人体皮肤转录组分析。

Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids.

机构信息

Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi, 321-3497, Japan.

出版信息

Commun Biol. 2022 Mar 9;5(1):215. doi: 10.1038/s42003-022-03154-w.

DOI:10.1038/s42003-022-03154-w
PMID:35264722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8907185/
Abstract

Non-invasive acquisition of mRNA data from the skin can be extremely useful for understanding skin physiology and diseases. Inspired by the holocrine process, in which the sebaceous glands secrete cell contents into the sebum, we focused on the possible presence of mRNAs in skin surface lipids (SSLs). We found that measurable levels of human mRNAs exist in SSLs, where the sebum protects them from degradation by RNases. The AmpliSeq transcriptome analysis was modified to measure SSL-RNA levels, and our results revealed that the SSL-RNAs predominantly comprised mRNAs derived from sebaceous glands, the epidermis, and hair follicles. Analysis of SSL-RNAs non-invasively collected from patients with atopic dermatitis revealed increased expression of inflammation-related genes and decreased expression of terminal differentiation-related genes, consistent with the results of previous reports. Further, we found that lipid synthesis-related genes were downregulated in the sebaceous glands of patients with atopic dermatitis. These results indicate that the analysis of SSL-RNAs is a promising strategy to understand the pathophysiology of skin diseases.

摘要

从皮肤无创获取 mRNA 数据对于理解皮肤生理学和疾病非常有用。受全浆分泌过程（即皮脂腺将细胞内容物分泌到皮脂中）的启发，我们专注于皮肤表面脂质 (SSL) 中可能存在的 mRNAs。我们发现，在皮脂保护其免受核糖核酸酶降解的情况下，SSL 中存在可测量水平的人类 mRNAs。我们修改了 AmpliSeq 转录组分析以测量 SSL-RNA 水平，结果表明 SSL-RNAs 主要由来自皮脂腺、表皮和毛囊的 mRNAs 组成。对从特应性皮炎患者无创收集的 SSL-RNAs 的分析显示，炎症相关基因的表达增加，而与终末分化相关的基因表达减少，这与之前的报告结果一致。此外，我们发现特应性皮炎患者的皮脂腺中与脂质合成相关的基因下调。这些结果表明，分析 SSL-RNAs 是一种很有前途的策略，可以帮助我们了解皮肤疾病的病理生理学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/0ab5fa9239e4/42003_2022_3154_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/d30ee462c7d6/42003_2022_3154_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/321fd66d403e/42003_2022_3154_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/46c74f2f9d5d/42003_2022_3154_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/0ab5fa9239e4/42003_2022_3154_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/d30ee462c7d6/42003_2022_3154_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/9e8518f2c900/42003_2022_3154_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/6de2a48466c3/42003_2022_3154_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/321fd66d403e/42003_2022_3154_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/46c74f2f9d5d/42003_2022_3154_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7563/8907185/0ab5fa9239e4/42003_2022_3154_Fig7_HTML.jpg

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