Schüller-Ravoo Sigrid, Teixeira Sandra M, Papenburg Bernke, Stamatialis Dimitrios, Feijen Jan, Grijpma Dirk W
MIRA Institute for Biomedical Engineering and Technical Medicine, and Department of Biomaterials Science and Technology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
MIRA Institute for Biomedical Engineering and Technical Medicine, and Department of Membrane Science and Technology, University of Twente, P.O. Box 217, 7500, AE, Enschede, The Netherlands.
Chemphyschem. 2018 Aug 17;19(16):2085-2092. doi: 10.1002/cphc.201701308. Epub 2018 Feb 28.
Photo-crosslinkable poly(trimethylene carbonate) (PTMC) macromers were used to fabricate microstructured surfaces. Microstructured PTMC surfaces were obtained by hot embossing the macromer against structured silicon masters and subsequent photo-crosslinking, resulting in network formation. The microstructures of the master could be precisely replicated, limiting the shrinkage. Microstructured PTMC was investigated for use in two different applications: as stamping material to transfer a model protein to another surface and as structured substrate for cell culture. Using the flexible and elastic materials as stamps, bovine serum albumin labelled with fluorescein isothiocyanate was patterned on glass surfaces. In cell culture experiments, the behavior of human mesenchymal stem cells on nonstructured and microstructured PTMC surfaces was investigated. The cells strongly adhered to the PTMC surfaces and proliferated well. Compared to poly(dimethylsiloxane) (PDMS), which is commonly used in soft lithography, the PTMC networks offer significant advantages. They show better compatibility with cells, are biodegradable, and have much better mechanical properties. Both materials are transparent, flexible, and elastic at room temperature, but the tear resistance of PTMC networks is much higher than that of PDMS. Thus, PTMC might be an alternative material to PDMS in the fields of biology, medicine, and tissue engineering, in which microfabricated devices are increasingly being applied.
可光交联的聚碳酸三亚甲基酯（PTMC）大分子单体被用于制造微结构表面。通过将大分子单体热压印在结构化的硅模板上并随后进行光交联，得到了微结构PTMC表面，从而形成网络结构。模板的微结构能够被精确复制，限制了收缩。对微结构PTMC在两种不同应用中的用途进行了研究：作为将模型蛋白转移到另一个表面的压印材料以及作为细胞培养的结构化基质。使用柔性且有弹性的材料作为印章，将异硫氰酸荧光素标记的牛血清白蛋白图案化在玻璃表面上。在细胞培养实验中，研究了人间充质干细胞在非结构化和微结构化PTMC表面上的行为。细胞强烈粘附于PTMC表面并增殖良好。与软光刻中常用的聚二甲基硅氧烷（PDMS）相比，PTMC网络具有显著优势。它们与细胞表现出更好的相容性，可生物降解，并且具有更好的机械性能。两种材料在室温下都是透明、柔性且有弹性的，但PTMC网络的抗撕裂性远高于PDMS。因此，在生物学、医学和组织工程领域中，微制造设备越来越多地被应用，PTMC可能是PDMS的替代材料。
